Anti-BEND3 antibody (STJA0008545)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
ImmunogenSynthesized peptide derived from human BEND3
General Information
| Short Description | Rabbit polyclonal anti-BEN domain-containing protein 3 for use in WB in Human and Mouse samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB |
| Host | Rabbit |
| Reactivity | Human/Mouse |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | 1 mg/mL |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Dilution Range | WB 1:500-2000 |
| Formulation | Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide. |
| Storage Instruction | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | BEND3 |
| Gene ID | 57673 |
| Uniprot ID | BEND3_HUMAN |
| Immunogen | Synthesized peptide derived from human BEND3 |
| Specificity | This antibody detects endogenous levels of BEND3 at Human, Mouse |
Additional Info
| Post Translational Modifications | Sumoylated at Lys-20 by SUMO1 and at Lys-512 by SUMO1, SUMO2 and SUMO3. Sumoylation probably occurs sequentially, with that of Lys-20 preceding that of Lys-512. It does not alter association with heterochromatin, but is required for the repression of transcription. |
| Function | Transcriptional repressor which associates with the NoRC (nucleolar remodeling complex) complex and plays a key role in repressing rDNA transcription. The sumoylated form modulates the stability of the NoRC complex component BAZ2A/TIP5 by controlling its USP21-mediated deubiquitination. Binds to unmethylated major satellite DNA and is involved in the recruitment of the Polycomb repressive complex 2 (PRC2) to major satellites. Stimulates the ERCC6L translocase and ATPase activities. |
| Protein Name | Ben Domain-Containing Protein 3 |
| Database Links | |
| Cellular Localisation | NucleusNucleolusIn The NucleusObserved In Heterochromatic Foci Containing Cbx1Cbx3Cbx5 And Histone H3 Trimethylated At 'Lys-9'Released From Chromatin During DecondensationAssociation With Heterochromatin Does Not Depend On Sumoylation |
| Alternative Antibody Names | Anti-Ben Domain-Containing Protein 3 antibodyAnti-BEND3 antibodyAnti-KIAA1553 antibody |
Information sourced from Uniprot.org