• Immunofluorescence analysis of HeLa using Beclin 1 Rabbit monoclonal antibody (STJ11104238) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of C6 using Beclin 1 Rabbit monoclonal antibody (STJ11104238) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
  • Western blot analysis of extracts from wild type (WT) and Beclin 1 knockout (KO) 293T cells, using Beclin 1 antibody (STJ11104238) at 1:20000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.
  • Western blot analysis of extracts of various cell lines, using Beclin 1 antibody (STJ11104238) at 1:10000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Anti-Beclin 1 antibody (1-280) [S4238RM] (STJ11104238)

SKU:
STJ11104238

£151.50 - £666.50
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Host: Rabbit
Applications: WB/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit monoclonal antibody anti-Beclin 1 (1-280) is suitable for use in Western Blot and Immunofluorescence research applications.
Clonality: Monoclonal
Clone ID: S4238RM
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.05% Proclin300, 0.05% BSA, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:2000-1:10000
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Immunogen Region: 1-280
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-280 of human Beclin 1 (NP_003757.1).
Immunogen Sequence: MEGSKTSNNSTMQVSFVCQR CSQPLKLDTSFKILDRVTIQ ELTAPLLTTAQAKPGETQEE ETNSGEEPFIETPRQDGVSR RFIPPARMMSTESANSFTLI GEASDGGTMENLSRRLKVTG DLFDIMSGQTDVDHPLCEEC TDTLLDQLDTQLNVTENECQ NYKRCLEILEQMNEDDSEQL QMELKELALEEERLIQELED VEKNRKIVAENLEKVQAEAE RLDQEEAQYQREYSEFKRQ
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance