• Western blot analysis of various lysates using BDNF Mouse monoclonal antibody (STJ11100099) at 1:10000 dilution. Secondary antibody: HRP Goat Anti-Mouse IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.

Anti-BDNF antibody (20-247) [S9MM] (STJ11100099)

SKU:
STJ11100099

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Host: Mouse
Applications: WB
Reactivity: Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Mouse monoclonal antibody anti-BDNF (20-247) is suitable for use in Western Blot research applications.
Clonality: Monoclonal
Clone ID: S9MM
Conjugation: Unconjugated
Isotype: lgG2ak
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:2000-1:10000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: BDNF
Gene ID: 627
Uniprot ID: BDNF_HUMAN
Immunogen Region: 20-247
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 20-247 of human BDNF (P23560).
Immunogen Sequence: PMKEANIRGQGGLAYPGVRT HGTLESVNGPKAGSRGLTSL ADTFEHVIEELLDEDQKVRP NEENNKDADLYTSRVMLSSQ VPLEPPLLFLLEEYKNYLDA ANMSMRVRRHSDPARRGELS VCDSISEWVTAADKKTAVDM SGGTVTVLEKVPVSKGQLKQ YFYETKCNPMGYTKEGCRGI DKRHWNSQCRTTQSYVRALT MDSKKRIGWRFIRIDTSCVC TLTIKRGR
Tissue Specificity Detected in blood plasma and in saliva (at protein level). Brain. Highly expressed in hippocampus, amygdala, cerebral cortex and cerebellum. Also expressed in heart, lung, skeletal muscle, testis, prostate and placenta.
Post Translational Modifications BDNF precursor form: N-glycosylated and glycosulfated, contrary to mature BDNF. Mature BDNF is produced by proteolytic removal of the propeptide, catalyzed by a FURIN family member. In addition, the precursor form is proteolytically cleaved within the propeptide, but this is not an obligatory intermediate for the production of mature BDNF. Can be converted into mature BDNF by plasmin (PLG).
Function Important signaling molecule that activates signaling cascades downstream of NTRK2. During development, promotes the survival and differentiation of selected neuronal populations of the peripheral and central nervous systems. Participates in axonal growth, pathfinding and in the modulation of dendritic growth and morphology. Major regulator of synaptic transmission and plasticity at adult synapses in many regions of the CNS. The versatility of BDNF is emphasized by its contribution to a range of adaptive neuronal responses including long-term potentiation (LTP), long-term depression (LTD), certain forms of short-term synaptic plasticity, as well as homeostatic regulation of intrinsic neuronal excitability. BDNF precursor form: Important signaling molecule that activates signaling cascades downstream of NTRK2. Activates signaling cascades via the heterodimeric receptor formed by NGFR and SORCS2. Signaling via NGFR and SORCS2 plays a role in synaptic plasticity and long-term depression (LTD). Binding to NGFR and SORCS2 promotes neuronal apoptosis. Promotes neuronal growth cone collapse.
Protein Name Brain-Derived Neurotrophic Factor
Bdnf
Abrineurin Cleaved Into - Bdnf Precursor Form
Probdnf
Database Links Reactome: R-HSA-9022702
Reactome: R-HSA-9024909
Reactome: R-HSA-9026519
Reactome: R-HSA-9026527
Reactome: R-HSA-9028335
Reactome: R-HSA-9028731
Reactome: R-HSA-9032500
Reactome: R-HSA-9032759
Reactome: R-HSA-9032845
Reactome: R-HSA-9768919
Cellular Localisation Secreted
Bdnf Precursor Form: Secreted
A Proportion Of Bdnf Is Secreted As Immature Precursor (Probdnf)
Alternative Antibody Names Anti-Brain-Derived Neurotrophic Factor antibody
Anti-Bdnf antibody
Anti-Abrineurin Cleaved Into - Bdnf Precursor Form antibody
Anti-Probdnf antibody
Anti-BDNF antibody

Information sourced from Uniprot.org

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