Anti-BCKDHA antibody (276-445) (STJ111848)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
General Information
| Short Description | Rabbit polyclonal BCKDHA (276-445) antibody for use in WB, IF, ICC and ELISA in human, mouse and rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/IF/ICC/ELISA |
| Host | Rabbit |
| Reactivity | Human/Mouse/Rat |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | Lot specific |
| Purification | Affinity purification |
| Dilution Range | WB:1:500-1:2000IF/ICC:1:50-1:200ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Formulation | PBS with 0.02% Sodium Azide, 50% Glycerol, pH 7.3. |
| Storage Instruction | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | BCKDHA |
| Gene ID | 593 |
| Uniprot ID | ODBA_HUMAN |
| Immunogen Region | 276-445 |
| Immunogen Sequence | SEQYRGDGIAARGPGYGIMS IRVDGNDVFAVYNATKEARR RAVAENQPFLIEAMTYRIGH HSTSDDSSAYRSVDEVNYWD KQDHPISRLRHYLLSQGWWD EEQEKAWRKQSRRKVMEAFE QAERKPKPNPNLLFSDVYQE MPAQLRKQQESLARHLQTYG EHYPLDHFDK |
| Specificity | Recombinant fusion protein containing a sequence corresponding to amino acids 276-445 of human BCKDHA (NP_000700.1). |
Additional Info
| Post Translational Modifications | Phosphorylated at Ser-337 by BCKDK and dephosphorylated by protein phosphatase PPM1K. |
| Function | Together with BCKDHB forms the heterotetrameric E1 subunit of the mitochondrial branched-chain alpha-ketoacid dehydrogenase (BCKD) complex. The BCKD complex catalyzes the multi-step oxidative decarboxylation of alpha-ketoacids derived from the branched-chain amino-acids valine, leucine and isoleucine producing CO2 and acyl-CoA which is subsequently utilized to produce energy. The E1 subunit catalyzes the first step with the decarboxylation of the alpha-ketoacid forming an enzyme-product intermediate. A reductive acylation mediated by the lipoylamide cofactor of E2 extracts the acyl group from the E1 active site for the next step of the reaction. |
| Protein Name | 2-Oxoisovalerate Dehydrogenase Subunit Alpha - MitochondrialBranched-Chain Alpha-Keto Acid Dehydrogenase E1 Component Alpha ChainBckde1aBckdh E1-Alpha |
| Database Links | Reactome: R-HSA-70895Reactome: R-HSA-9859138Reactome: R-HSA-9865113Reactome: R-HSA-9865125Reactome: R-HSA-9907570Reactome: R-HSA-9912481Reactome: R-HSA-9912529 |
| Cellular Localisation | Mitochondrion Matrix |
| Alternative Antibody Names | Anti-2-Oxoisovalerate Dehydrogenase Subunit Alpha - Mitochondrial antibodyAnti-Branched-Chain Alpha-Keto Acid Dehydrogenase E1 Component Alpha Chain antibodyAnti-Bckde1a antibodyAnti-Bckdh E1-Alpha antibodyAnti-BCKDHA antibody |
Information sourced from Uniprot.org