• Western blot analysis of extracts of various cell lines, using BCCIP antibody (STJ111319) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.
  • Immunohistochemistry analysis of paraffin-embedded human breast using BCCIP antibody (STJ111319) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry of paraffin-embedded human breast using BCCIP antibody (STJ111319) at dilution of 1:100 (40x lens).
  • Western blot analysis of extracts of various cell lines, using BCCIP antibody (STJ111319) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-BCCIP antibody (1-314) (STJ111319)

SKU:
STJ111319

£112.50 - £522.50
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Host: Rabbit
Applications: WB/IHC
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-BCCIP (1-314) is suitable for use in Western Blot and Immunohistochemistry research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IHC-P 1:100-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: BCCIP
Gene ID: 56647
Uniprot ID: BCCIP_HUMAN
Immunogen Region: 1-314
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-314 of human BCCIP (NP_510868.1).
Immunogen Sequence: MASRSKRRAVESGVPQPPDP PVQRDEEEEKEVENEDEDDD DSDKEKDEEDEVIDEEVNIE FEAYSLSDNDYDGIKKLLQQ LFLKAPVNTAELTDLLIQQN HIGSVIKQTDVSEDSNDDMD EDEVFGFISLLNLTERKGTQ CVEQIQELVLRFCEKNCEKS MVEQLDKFLNDTTKPVGLLL SERFINVPPQIALPMYQQLQ KELAGAHRTNKPCGKCYFYL LISKTFVEAGKNNSKKKPS
Tissue Specificity Expressed at high levels in testis and skeletal muscle and at lower levels in brain, heart, kidney, liver, lung, ovary, pancreas, placenta, and spleen.
Function During interphase, required for microtubule organizing and anchoring activities. During mitosis, required for the organization and stabilization of the spindle pole. Isoform 2/alpha is particularly important for the regulation of microtubule anchoring, microtubule stability, spindle architecture and spindle orientation, compared to isoform 1/beta. May promote cell cycle arrest by enhancing the inhibition of CDK2 activity by CDKN1A. May be required for repair of DNA damage by homologous recombination in conjunction with BRCA2. May not be involved in non-homologous end joining (NHEJ).
Protein Name Brca2 And Cdkn1a-Interacting Protein
P21- And Cdk-Associated Protein 1
Protein Tok-1
Cellular Localisation Nucleus
Cytoplasm
Cytoskeleton
Microtubule Organizing Center
Centrosome
Centriole
Spindle Pole
Colocalizes With Brca2 In Discrete Nuclear Foci
In Interphase
Preferential Localizes To The Mother Centriole
Recruited To The Spindle Pole Matrix And Centrosome By Microtubules And Dynein/Dynactin Activity
Isoform 1: Cytoplasm
Isoform 1/Beta Tends To Be Less Abundant At
And Less Strongly Associated With
Centrosomes Than Isoform 2/Alpha
Isoform 2: Cytoplasm
Isoform 2/Alpha Tends To Be More Abundant At
And More Strongly Associated With
Centrosomes Than Isoform 1/Beta
Alternative Antibody Names Anti-Brca2 And Cdkn1a-Interacting Protein antibody
Anti-P21- And Cdk-Associated Protein 1 antibody
Anti-Protein Tok-1 antibody
Anti-BCCIP antibody
Anti-TOK1 antibody

Information sourced from Uniprot.org

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