• Confocal immunofluorescence analysis of U2OS cells using BCAS2 Polyclonal Antibody (STJ26653) at dilution of 1:100. Blue: DAPI for nuclear staining.
  • Immunohistochemistry analysis of paraffin-embedded mouse brain using BCAS2 Rabbit polyclonal antibody (STJ26653) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded human esophageal cancer using BCAS2 Rabbit polyclonal antibody (STJ26653) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded rat kidney using BCAS2 Rabbit polyclonal antibody (STJ26653) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of extracts of various cell lines, using BCAS2 antibody (STJ26653) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-BCAS2 antibody (1-225) (STJ26653)

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STJ26653

£112.50 - £522.50
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Host: Rabbit
Applications: WB/IHC/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-BCAS2 (1-225) is suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IHC-P 1:50-1:200
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: BCAS2
Gene ID: 10286
Uniprot ID: SPF27_HUMAN
Immunogen Region: 1-225
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-225 of human BCAS2 (NP_005863.1).
Immunogen Sequence: MAGTGLVAGEVVVDALPYFD QGYEAPGVREAAAALVEEET RRYRPTKNYLSYLTAPDYSA FETDIMRNEFERLAARQPIE LLSMKRYELPAPSSGQKNDI TAWQECVNNSMAQLEHQAVR IENLELMSQHGCNAWKVYNE NLVHMIEHAQKELQKLRKHI QDLNWQRKNMQLTAGSKLRE MESNWVSLVSKNYEIERTIV QLENEIYQIKQQHGEANKEN IRQDF
Tissue Specificity Ubiquitously expressed.
Function Required for pre-mRNA splicing as component of the activated spliceosome. Component of the PRP19-CDC5L complex that forms an integral part of the spliceosome and is required for activating pre-mRNA splicing. May have a scaffolding role in the spliceosome assembly as it contacts all other components of the core complex. The PRP19-CDC5L complex may also play a role in the response to DNA damage (DDR).
Protein Name Pre-Mrna-Splicing Factor Spf27
Breast Carcinoma-Amplified Sequence 2
Dna Amplified In Mammary Carcinoma 1 Protein
Spliceosome-Associated Protein Spf 27
Database Links Reactome: R-HSA-72163
Cellular Localisation Nucleus
Nucleolus
Alternative Antibody Names Anti-Pre-Mrna-Splicing Factor Spf27 antibody
Anti-Breast Carcinoma-Amplified Sequence 2 antibody
Anti-Dna Amplified In Mammary Carcinoma 1 Protein antibody
Anti-Spliceosome-Associated Protein Spf 27 antibody
Anti-BCAS2 antibody
Anti-DAM1 antibody

Information sourced from Uniprot.org

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