Anti-BCAR3 antibody (761-810 aa) (STJ91828)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
ImmunogenThe antiserum was produced against synthesized peptide derived from the human BCAR3 at the amino acid range 761-810
General Information
| Short Description | Rabbit polyclonal anti-Breast cancer anti-estrogen resistance protein 3 (761-810 aa) for use in WB, IHC, IF and ELISA in Human, Rat and Mouse samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/IHC/IF/ELISA |
| Host | Rabbit |
| Reactivity | Human/Rat/Mouse |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | 1 mg/mL |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Dilution Range | WB 1:500-1:2000IHC 1:100-1:300IF 1:200-1:1000ELISA 1:10000 |
| Formulation | Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide. |
| Storage Instruction | Store at-20ยฐC for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | BCAR3 |
| Gene ID | 8412 |
| Uniprot ID | BCAR3_HUMAN |
| Immunogen | The antiserum was produced against synthesized peptide derived from the human BCAR3 at the amino acid range 761-810 |
| Immunogen Region | 761-810 aa |
| Specificity | BCAR3 Polyclonal Antibody detects endogenous levels of BCAR3 protein. |
Additional Info
| Post Translational Modifications | Phosphorylated on tyrosine residues. |
| Function | Acts as an adapter protein downstream of several growth factor receptors to promote cell proliferation, migration, and redistribution of actin fibers. Specifically involved in INS/insulin signaling pathway by mediating MAPK1/ERK2-MAPK3/ERK1 activation and DNA synthesis. Promotes insulin-mediated membrane ruffling. In response to vasoconstrictor peptide EDN1, involved in the activation of RAP1 downstream of PTK2B via interaction with phosphorylated BCAR1. Inhibits cell migration and invasion via regulation of TGFB-mediated matrix digestion, actin filament rearrangement, and inhibition of invadopodia activity. May inhibit TGFB-SMAD signaling, via facilitating BCAR1 and SMAD2 and/or SMAD3 interaction. Regulates EGF-induced DNA synthesis. Required for the maintenance of ocular lens morphology and structural integrity, potentially via regulation of focal adhesion complex signaling. Acts upstream of PTPRA to regulate the localization of BCAR1 and PTPRA to focal adhesions, via regulation of SRC-mediated phosphorylation of PTPRA. Positively regulates integrin-induced tyrosine phosphorylation of BCAR1. Acts as a guanine nucleotide exchange factor (GEF) for small GTPases RALA, RAP1A and RRAS. However, in a contrasting study, lacks GEF activity towards RAP1. |
| Protein Name | Breast Cancer Anti-Estrogen Resistance Protein 3Novel Sh2-Containing Protein 2Sh2 Domain-Containing Protein 3b |
| Database Links | |
| Cellular Localisation | CytoplasmCell JunctionFocal AdhesionLocalization To Focal Adhesions Depends On Interaction With Ptpra |
| Alternative Antibody Names | Anti-Breast Cancer Anti-Estrogen Resistance Protein 3 antibodyAnti-Novel Sh2-Containing Protein 2 antibodyAnti-Sh2 Domain-Containing Protein 3b antibodyAnti-BCAR3 antibodyAnti-NSP2 antibodyAnti-SH2D3B antibodyAnti-UNQ271 antibodyAnti-PRO308 antibody |
Information sourced from Uniprot.org