• Western blot analysis of extracts of various cell lines, using BBOX1 antibody (STJ28373) at 1:500 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.
  • Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with IF staining protocol. Immunofluorescence analysis of mouse kidney cells using BBOX1 rabbit polyclonal antibody (STJ28373) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Anti-BBOX1 antibody (1-180) (STJ28373)

SKU:
STJ28373

£112.50 - £524.50
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Host: Rabbit
Applications: WB/IF/ICC/ELISA
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH 7.3.
Purification: Affinity purification
Concentration: Lot specific
Dilution Range: WB:1:100-1:500
IF/ICC:1:50-1:200
ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements.
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: BBOX1
Gene ID: 8424
Uniprot ID: BODG_HUMAN
Immunogen Region: 1-180
Specificity: Recombinant fusion protein containing a sequence corresponding to amino acids 1-180 of human BBOX1 (NP_003977.1).
Immunogen Sequence: MACTIQKAEALDGAHLMQIL WYDEEESLYPAVWLRDNCPC SDCYLDSAKARKLLVEALDV NIGIKGLIFDRKKVYITWPD EHYSEFQADWLKKRCFSKQA RAKLQRELFFPECQYWGSEL QLPTLDFEDVLRYDEHAYKW LSTLKKVGIVRLTGASDKPG EVSKLGKRMGFLYLTFYGHT
Tissue Specificity Highly expressed in kidney.moderately expressed in liver.very low expression in brain.
Function Catalyzes the formation of L-carnitine from gamma-butyrobetaine.
Protein Name Gamma-Butyrobetaine Dioxygenase
Gamma-Butyrobetaine Hydroxylase
Gamma-Bbh
Gamma-Butyrobetaine -2-Oxoglutarate Dioxygenase
Database Links Reactome: R-HSA-71262
Cellular Localisation Cytoplasm
Alternative Antibody Names Anti-Gamma-Butyrobetaine Dioxygenase antibody
Anti-Gamma-Butyrobetaine Hydroxylase antibody
Anti-Gamma-Bbh antibody
Anti-Gamma-Butyrobetaine -2-Oxoglutarate Dioxygenase antibody
Anti-BBOX1 antibody
Anti-BBH antibody
Anti-BBOX antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance