• Immunofluorescence analysis of NIH-3T3 cells using PUMA Rabbit polyclonal antibody (STJ119319) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Western blot analysis of various lysates using PUMA Rabbit polyclonal antibody (STJ119319) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Anti-BBC3 antibody (50-131) (STJ119319)

SKU:
STJ119319

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Host: Rabbit
Applications: WB/IHC/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-BBC3 (50-131) is suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IHC-P 1:50-1:100
IF/ICC 1:50-1:100
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: BBC3
BBC3
Gene ID: 27113
27113
Uniprot ID: BBC3_HUMAN
BBC3B_HUMAN
Immunogen Region: 50-131
Immunogen: A synthetic peptide corresponding to a sequence within amino acids 50-131 of human PUMA (NP_001120713.1).
Immunogen Sequence: PGALAGGPTQAAPGVRGEEE QWAREIGAQLRRMADDLNAQ YERRRQEEQQRHRPSPWRVL YNLIMGLLPLPRGHRAPEME PN
Tissue Specificity
Alternative Antibody Names Anti-Bcl-2-Binding Component 3 - Isoforms 1/2 antibody
Anti-Jfy-1 antibody
Anti-P53 Up-Regulated Modulator Of Apoptosis antibody
Anti-BBC3 antibody
Anti-PUMA antibody
Anti-Bcl-2-Binding Component 3 - Isoforms 3/4 antibody
Anti-Jfy-1 antibody
Anti-P53 Up-Regulated Modulator Of Apoptosis antibody
Anti-BBC3 antibody
Anti-PUMA antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance