| Host: |
Rabbit |
| Applications: |
WB/IHC/IF |
| Reactivity: |
Human/Mouse/Rat |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Rabbit polyclonal antibody anti-BAP1 (1-270) is suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence research applications. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3. |
| Purification: |
Affinity purification |
| Dilution Range: |
WB 1:500-1:1000IHC-P 1:50-1:200IF/ICC 1:50-1:200 |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: |
BAP1 |
| Gene ID: |
8314 |
| Uniprot ID: |
BAP1_HUMAN |
| Immunogen Region: |
1-270 |
| Immunogen: |
Recombinant fusion protein containing a sequence corresponding to amino acids 1-270 of human BAP1 (NP_004647.1). |
| Immunogen Sequence: |
MNKGWLELESDPGLFTLLVE DFGVKGVQVEEIYDLQSKCQ GPVYGFIFLFKWIEERRSRR KVSTLVDDTSVIDDDIVNNM FFAHQLIPNSCATHALLSVL LNCSSVDLGPTLSRMKDFTK GFSPESKGYAIGNAPELAKA HNSHARPEPRHLPEKQNGLS AVRTMEAFHFVSYVPITGRL FELDGLKVYPIDHGPWGEDE EWTDKARRVIMERIGLATAG EPYHDIRFNLMAVVPDRRI |
| Tissue Specificity | Highly expressed in testis, placenta and ovary. Expressed in breast. |
| Post Translational Modifications | Ubiquitinated: monoubiquitinated at multiple site of its nuclear localization signal (NLS) BY UBE2O, leading to cytoplasmic retention. Able to mediate autodeubiquitination via intramolecular interactions to couteract cytoplasmic retention. |
| Function | Deubiquitinating enzyme that plays a key role in chromatin by mediating deubiquitination of histone H2A and HCFC1. Catalytic component of the PR-DUB complex, a complex that specifically mediates deubiquitination of histone H2A monoubiquitinated at 'Lys-119' (H2AK119ub1). Does not deubiquitinate monoubiquitinated histone H2B. Acts as a regulator of cell growth by mediating deubiquitination of HCFC1 N-terminal and C-terminal chains, with some specificity toward 'Lys-48'-linked polyubiquitin chains compared to 'Lys-63'-linked polyubiquitin chains. Deubiquitination of HCFC1 does not lead to increase stability of HCFC1. Interferes with the BRCA1 and BARD1 heterodimer activity by inhibiting their ability to mediate ubiquitination and autoubiquitination. It however does not mediate deubiquitination of BRCA1 and BARD1. Able to mediate autodeubiquitination via intramolecular interactions to couteract monoubiquitination at the nuclear localization signal (NLS), thereby protecting it from cytoplasmic sequestration. Acts as a tumor suppressor. |
| Protein Name | Ubiquitin Carboxyl-Terminal Hydrolase Bap1Brca1-Associated Protein 1Cerebral Protein 6 |
| Database Links | Reactome: R-HSA-5689603Reactome: R-HSA-5693565 |
| Cellular Localisation | CytoplasmNucleusMainly NuclearBinds To ChromatinLocalizes To The Cytoplasm When Monoubiquitinated By The E2/E3 Hybrid Ubiquitin-Protein Ligase Ube2o |
| Alternative Antibody Names | Anti-Ubiquitin Carboxyl-Terminal Hydrolase Bap1 antibodyAnti-Brca1-Associated Protein 1 antibodyAnti-Cerebral Protein 6 antibodyAnti-BAP1 antibodyAnti-KIAA0272 antibodyAnti-hucep-6 antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
