| Short Description : | Rabbit polyclonal BAG3 (380-575) antibody for use in WB, IHC-P and ELISA in human, mouse and rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications: | WB/IHC-P/ELISA |
| Host: | Rabbit |
| Reactivity: | Human/Mouse/Rat |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Clonality : | Polyclonal |
| Isotype: | IgG |
| Conjugation: | Unconjugated |
| Concentration: | Lot specific |
| Purification: | Affinity purification |
| Dilution Range: | WB:1:500-1:2000IHC-P:1:50-1:100ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Formulation: | PBS with 0.01% Thimerosal, 50% Glycerol, pH 7.3. |
| Storage Instruction: | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: | BAG3 |
| Gene ID: | 9531 |
| Uniprot ID: | BAG3_HUMAN |
| Immunogen Region: | 380-575 |
| Immunogen Sequence: | PSAVPSSPKSVATEERAAPS TAPAEATPPKPGEAEAPPKH PGVLKVEAILEKVQGLEQAV DNFEGKKTDKKYLMIEEYLT KELLALDSVDPEGRADVRQA RRDGVRKVQTILEKLEQKAI DVPGQVQVYELQPSNLEADQ PLQAIMEMGAVAADKGKKNA GNAEDPHTETQQPEATAAAT SNPSSMTDTPGNPAAP |
| Specificity: | Recombinant fusion protein containing a sequence corresponding to amino acids 380-575 of human BAG3 (NP_004272.2). |
| Function | Co-chaperone and adapter protein that connects different classes of molecular chaperones including heat shock proteins 70 (HSP70s), e.g. HSPA1A/HSP70 or HSPA8/HSC70, and small heat shock proteins (sHSPs), e.g. HSPB8. Acts as a nucleotide-exchange factor (NEF) promoting the release of ADP from HSP70s, thereby triggering client protein release. Nucleotide release is mediated via BAG3 binding to the nucleotide-binding domain (NBD) of HSP70s, whereas client release is mediated via binding to the substrate-binding domain (SBD). Has anti-apoptotic activity. Plays a role in the HSF1 nucleocytoplasmic transport. |
| Protein Name | Bag Family Molecular Chaperone Regulator 3Bag-3Bcl-2-Associated Athanogene 3Bcl-2-Binding Protein BisDocking Protein Cair-1 |
| Database Links | Reactome: R-HSA-3371453 |
| Cellular Localisation | NucleusCytoplasmColocalizes With Hsf1 To The Nucleus Upon Heat Stress |
| Alternative Antibody Names | Anti-Bag Family Molecular Chaperone Regulator 3 antibodyAnti-Bag-3 antibodyAnti-Bcl-2-Associated Athanogene 3 antibodyAnti-Bcl-2-Binding Protein Bis antibodyAnti-Docking Protein Cair-1 antibodyAnti-BAG3 antibodyAnti-BIS antibody |
Information sourced from Uniprot.org
