Anti-Bad antibody (STJA0037831)
SPECIFICATIONS
ClonalityMonoclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
ImmunogenA synthesized peptide derived from human Bad
General Information
| Short Description | Rabbit monoclonal anti-Bad for use in IHC-P in Human samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | IHC-P |
| Host | Rabbit |
| Reactivity | Human |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Monoclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Purification | Affinity Purified |
| Dilution Range | IHC 1:100-1:200 |
| Formulation | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide, pH 7.3. |
| Storage Instruction | Store at 4°C short term. Aliquot and store at-20°C long term. Avoid freeze/thaw cycles. |
Target Information
| Gene Symbol | BAD |
| Gene ID | 572 |
| Uniprot ID | BAD_HUMAN |
| Immunogen | A synthesized peptide derived from human Bad |
Additional Info
| Tissue Specificity | Expressed in a wide variety of tissues. |
| Post Translational Modifications | Phosphorylated on one or more of Ser-75, Ser-99, Ser-118 and Ser-134 in response to survival stimuli, which blocks its pro-apoptotic activity. Phosphorylation on Ser-99 or Ser-75 promotes heterodimerization with 14-3-3 proteins. This interaction then facilitates the phosphorylation at Ser-118, a site within the BH3 motif, leading to the release of Bcl-X(L) and the promotion of cell survival. Ser-99 is the major site of AKT/PKB phosphorylation, Ser-118 the major site of protein kinase A (CAPK) phosphorylation. Phosphorylation at Ser-99 by PKB/AKT1 is almost completely blocked by the apoptotic C-terminus cleavage product of PKN2 generated by caspases-3 activity during apoptosis. Methylation at Arg-94 and Arg-96 by PRMT1 inhibits Akt-mediated phosphorylation at Ser-99. |
| Function | Promotes cell death. Successfully competes for the binding to Bcl-X(L), Bcl-2 and Bcl-W, thereby affecting the level of heterodimerization of these proteins with BAX. Can reverse the death repressor activity of Bcl-X(L), but not that of Bcl-2. Appears to act as a link between growth factor receptor signaling and the apoptotic pathways. |
| Protein Name | Bcl2-Associated Agonist Of Cell DeathBadBcl-2-Binding Component 6Bcl-2-Like Protein 8Bcl2-L-8Bcl-Xl/Bcl-2-Associated Death PromoterBcl2 Antagonist Of Cell Death |
| Database Links | Reactome: R-HSA-111447Reactome: R-HSA-111453Reactome: R-HSA-193648Reactome: R-HSA-198323Reactome: R-HSA-5674400 |
| Cellular Localisation | Mitochondrion Outer MembraneCytoplasmColocalizes With Hif3a In The CytoplasmUpon PhosphorylationLocates To The Cytoplasm |
| Alternative Antibody Names | Anti-Bcl2-Associated Agonist Of Cell Death antibodyAnti-Bad antibodyAnti-Bcl-2-Binding Component 6 antibodyAnti-Bcl-2-Like Protein 8 antibodyAnti-Bcl2-L-8 antibodyAnti-Bcl-Xl/Bcl-2-Associated Death Promoter antibodyAnti-Bcl2 Antagonist Of Cell Death antibodyAnti-BAD antibodyAnti-BBC6 antibodyAnti-BCL2L8 antibody |
Information sourced from Uniprot.org