Anti-B-Raf-45221 antibody (N-Term) (STJA0041363)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
ImmunogenA synthetic peptide (coupled to KLH) corresponding to amino acid residues in the N-terminus of human B-Raf. This sequence has high homology with similar regions in rat and mouse B-Raf, and has low homology to other Raf family members.
General Information
| Short Description | Rabbit polyclonal anti-B-Raf-45221 (N-Term) for use in ELISA and WB in Human, Mouse and Rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | ELISA/WB |
| Host | Rabbit |
| Reactivity | Human/Mouse/Rat |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Purification | Antigen Affinity Purified |
| Dilution Range | WB 1:1000 |
| Formulation | PBS + 1 mg/ml BSA, 0.05% NaN3 and 50% glycerol |
| Storage Instruction | Storage at-20ยฐC is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at-20ยฐC. |
Target Information
| Gene Symbol | BRAF |
| Gene ID | 673 |
| Uniprot ID | BRAF_HUMAN |
| Immunogen | A synthetic peptide (coupled to KLH) corresponding to amino acid residues in the N-terminus of human B-Raf. This sequence has high homology with similar regions in rat and mouse B-Raf, and has low homology to other Raf family members. |
| Immunogen Region | N-Term |
| Immunogen Sequence | Human |
| Specificity | The antibody detects 105 and 95 kDa* bands corresponding to the molecular mass of B-Raf. The doublet observed in Jurkat cells may represent multipe isoforms of the B-Raf, since all bands are blocked specifically with B-Raf (N-terminus) peptide. |
Additional Info
| Tissue Specificity | Brain and testis. |
| Post Translational Modifications | Phosphorylation at Ser-365 by SGK1 inhibits its activity (Ref.8, PubMed:11410590). Phosphorylation at Thr-753 by MAPK1. Dephosphorylation of Ser-365 by the SHOC2-MRAS-PP1c (SMP) complex consisting of SHOC2, GTP-bound M-Ras/MRAS and the catalytic subunit of protein phosphatase 1 (PPP1CA, PPP1CB or PPP1CC).this relieves inactivation and stimulates kinase activity. Methylation at Arg-671 decreases stability and kinase activity. Ubiquitinated by RNF149.which leads to proteasomal degradation. Polyubiquitinated at Lys-578 in response to EGF. |
| Function | Protein kinase involved in the transduction of mitogenic signals from the cell membrane to the nucleus (Probable). Phosphorylates MAP2K1, and thereby activates the MAP kinase signal transduction pathway. Phosphorylates PFKFB2. May play a role in the postsynaptic responses of hippocampal neurons. |
| Protein Name | Serine/Threonine-Protein Kinase B-RafProto-Oncogene B-RafP94V-Raf Murine Sarcoma Viral Oncogene Homolog B1 |
| Database Links | Reactome: R-HSA-1295596Reactome: R-HSA-170968Reactome: R-HSA-170984Reactome: R-HSA-187706Reactome: R-HSA-5673000Reactome: R-HSA-5674135Reactome: R-HSA-5674499Reactome: R-HSA-5675221Reactome: R-HSA-6802946Reactome: R-HSA-6802948Reactome: R-HSA-6802952Reactome: R-HSA-6802955Reactome: R-HSA-9649948Reactome: R-HSA-9656223Reactome: R-HSA-9726840Reactome: R-HSA-9726842 |
| Cellular Localisation | NucleusCytoplasmCell MembraneColocalizes With Rgs14 And Raf1 In Both The Cytoplasm And Membranes |
| Alternative Antibody Names | Anti-Serine/Threonine-Protein Kinase B-Raf antibodyAnti-Proto-Oncogene B-Raf antibodyAnti-P94 antibodyAnti-V-Raf Murine Sarcoma Viral Oncogene Homolog B1 antibodyAnti-BRAF antibodyAnti-BRAF1 antibodyAnti-RAFB1 antibody |
Information sourced from Uniprot.org