Anti-AXIN2 antibody (400-500) (STJ11102832)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
General Information
| Short Description | Rabbit polyclonal AXIN2 (400-500) antibody for use in WB and ELISA in mouse and rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/ELISA |
| Host | Rabbit |
| Reactivity | Mouse/Rat |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | Lot specific |
| Purification | Affinity purification |
| Dilution Range | WB:1:500-1:2000ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Formulation | PBS with 0.05% Proclin300, 50% Glycerol, pH 7.3. |
| Storage Instruction | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | AXIN2 |
| Gene ID | 8313 |
| Uniprot ID | AXIN2_HUMAN |
| Immunogen Region | 400-500 |
| Immunogen Sequence | EDEEREGSELTLNSREGAPT QHPLSLLPSGSYEEDPQTIL DDHLSRVLKTPGCQSPGVGR YSPRSRSPDHHHHHHSQYHS LLPPGGKLPPAAASPGACPL L |
| Specificity | A synthetic peptide corresponding to a sequence within amino acids 400-500 of human AXIN2 (NP_004646.3). |
Additional Info
| Tissue Specificity | Expressed in brain and lymphoblast. |
| Post Translational Modifications | Probably phosphorylated by GSK3B and dephosphorylated by PP2A. ADP-ribosylated by tankyrase TNKS and TNKS2. Poly-ADP-ribosylated protein is recognized by RNF146, followed by ubiquitination and subsequent activation of the Wnt signaling pathway. Ubiquitinated by RNF146 when poly-ADP-ribosylated, leading to its degradation and subsequent activation of the Wnt signaling pathway. Deubiquitinated by USP34, deubiquitinated downstream of beta-catenin stabilization step: deubiquitination is important Wnt signaling to positively regulate beta-catenin (CTNBB1)-mediated transcription. |
| Function | Inhibitor of the Wnt signaling pathway. Down-regulates beta-catenin. Probably facilitate the phosphorylation of beta-catenin and APC by GSK3B. |
| Protein Name | Axin-2Axin-Like ProteinAxilAxis Inhibition Protein 2Conductin |
| Database Links | Reactome: R-HSA-201681Reactome: R-HSA-4411364Reactome: R-HSA-4641257Reactome: R-HSA-5689880 |
| Cellular Localisation | Cytoplasm |
| Alternative Antibody Names | Anti-Axin-2 antibodyAnti-Axin-Like Protein antibodyAnti-Axil antibodyAnti-Axis Inhibition Protein 2 antibodyAnti-Conductin antibodyAnti-AXIN2 antibody |
Information sourced from Uniprot.org