Anti-Autophagy-related protein 9A antibody (C-Term) (STJA0040946)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeMixed
ImmunogenA synthetic peptide (CWHRRESDESGE) corresponding to the C-terminal of human APG9A (APG9L1) protein has been used as the immunogen. The peptide is homologous with the corresponding sequence derived from APG9A (APG9L1) protein in mouse, rat, S. cerevis
General Information
| Short Description | Rabbit polyclonal anti-Autophagy-related protein 9A (C-Term) for use in IHC-F and WB in Human and Rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | IHC-F/WB |
| Host | Rabbit |
| Reactivity | Human/Rat |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | Mixed |
| Conjugation | Unconjugated |
| Purification | Whole serum |
| Dilution Range | WB 1:100-1:3000IHC 1:100-1:3000 |
| Storage Instruction | Spin vial briefly before opening. Reconstitute in 100 µL sterile-filtered, ultrapure water. Centrifuge to remove any insoluble material. After reconstitution keep aliquots at-20°C for a higher stability, and at 2-8°C with an appropriate antibacterial |
Target Information
| Gene Symbol | ATG9A |
| Gene ID | 79065 |
| Uniprot ID | ATG9A_HUMAN |
| Immunogen | A synthetic peptide (CWHRRESDESGE) corresponding to the C-terminal of human APG9A (APG9L1) protein has been used as the immunogen. The peptide is homologous with the corresponding sequence derived from APG9A (APG9L1) protein in mouse, rat, S. cerevis |
| Immunogen Region | C-Term |
| Immunogen Sequence | Human |
| Specificity | IHC, WB and ELISA confirmed the specificity for ATG9A (APG9L1). Human, rat. Other species not yet tested. |
Additional Info
| Post Translational Modifications | Ufmylated in a DDRGK1 dependent manner. |
| Function | Phospholipid scramblase involved in autophagy by mediating autophagosomal membrane expansion. Cycles between the preautophagosomal structure/phagophore assembly site (PAS) and the cytoplasmic vesicle pool and supplies membrane for the growing autophagosome. Lipid scramblase activity plays a key role in preautophagosomal structure/phagophore assembly by distributing the phospholipids that arrive through ATG2 (ATG2A or ATG2B) from the cytoplasmic to the luminal leaflet of the bilayer, thereby driving autophagosomal membrane expansion. Also required to supply phosphatidylinositol 4-phosphate to the autophagosome initiation site by recruiting the phosphatidylinositol 4-kinase beta (PI4KB) in a process dependent on ARFIP2, but not ARFIP1. In addition to autophagy, also plays a role in necrotic cell death. |
| Protein Name | Autophagy-Related Protein 9aApg9-Like 1Matg9 |
| Database Links | Reactome: R-HSA-1632852Reactome: R-HSA-5205685 |
| Cellular Localisation | Preautophagosomal Structure MembraneMulti-Pass Membrane ProteinCytoplasmic VesicleAutophagosome MembraneGolgi ApparatusTrans-Golgi Network MembraneLate Endosome MembraneRecycling Endosome MembraneEndoplasmic Reticulum MembraneMitochondrion MembraneMainly Localizes To The Trans-Golgi Network (Tgn) And The Endosomal SystemCycles Between Them Though Vesicle TraffickingExport From The Tgn To Promote Formation Of Autophagosomes Is Mediated By The Ap-4 ComplexUnder Amino Acid Starvation Or Rapamycin TreatmentRedistributes To Preautophagosomal Structure/Phagophore Assembly Site (Pas)The Starvation-Induced Redistribution Depends On Ulk1Atg13As Well As Sh3glb1Upon Autophagy InductionA Small Portion Transiently Localizes To The Autophagic MembranesRecruited To Damaged Mitochondria During Mitophagy In A Rimoc1-Dependent Manner |
| Alternative Antibody Names | Anti-Autophagy-Related Protein 9a antibodyAnti-Apg9-Like 1 antibodyAnti-Matg9 antibodyAnti-ATG9A antibodyAnti-APG9L1 antibody |
Information sourced from Uniprot.org