| Host: | Goat |
| Applications: | Pep-ELISA/WB |
| Reactivity: | Human/Mouse/Rat/Dog |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description : | Goat polyclonal antibody anti-Aurora Kinase B (Internal) is suitable for use in ELISA and Western Blot research applications. |
| Clonality : | Polyclonal |
| Conjugation: | Unconjugated |
| Isotype: | IgG |
| Formulation: | 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. NA |
| Purification: | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Concentration: | 0.5 mg/mL |
| Dilution Range: | Peptide ELISA: antibody detection limit dilution 1:128000. WB: Approx 37kDa band observed in Human Thymus lysates and approx. 35kDa in Human Lymph Node, Spleen, Tonsil and Testis lysates (calculated MW of 39.3kDa according to NP_004208.2). Recomm |
| Storage Instruction: | Store at-20°C on receipt and minimise freeze-thaw cycles. |
| Gene Symbol: | AURKB |
| Gene ID: | 9212 |
| Uniprot ID: | AURKB_HUMAN |
| Immunogen Region: | Internal |
| Accession Number: | NP_004208.2 |
| Immunogen Sequence: | YKELQKSCTFDEQ |
| Post Translational Modifications | The phosphorylation of Thr-232 requires the binding to INCENP and occurs by means of an autophosphorylation mechanism. Thr-232 phosphorylation is indispensable for the AURKB kinase activity. Acetylated at Lys-215 by KAT5 at kinetochores, increasing AURKB activity and promoting accurate chromosome segregation in mitosis. Ubiquitinated by different BCR (BTB-CUL3-RBX1) E3 ubiquitin ligase complexes. Ubiquitinated by the BCR(KLHL9-KLHL13) E3 ubiquitin ligase complex, ubiquitination leads to removal from mitotic chromosomes and is required for cytokinesis. During anaphase, the BCR(KLHL21) E3 ubiquitin ligase complex recruits the CPC complex from chromosomes to the spindle midzone and mediates the ubiquitination of AURKB. Ubiquitination of AURKB by BCR(KLHL21) E3 ubiquitin ligase complex may not lead to its degradation by the proteasome. Deubiquitinated by USP35.inhibiting CDH1-mediated degradation of AURKB. |
| Function | Serine/threonine-protein kinase component of the chromosomal passenger complex (CPC), a complex that acts as a key regulator of mitosis. The CPC complex has essential functions at the centromere in ensuring correct chromosome alignment and segregation and is required for chromatin-induced microtubule stabilization and spindle assembly. Involved in the bipolar attachment of spindle microtubules to kinetochores and is a key regulator for the onset of cytokinesis during mitosis. Required for central/midzone spindle assembly and cleavage furrow formation. Key component of the cytokinesis checkpoint, a process required to delay abscission to prevent both premature resolution of intercellular chromosome bridges and accumulation of DNA damage: phosphorylates CHMP4C, leading to retain abscission-competent VPS4 (VPS4A and/or VPS4B) at the midbody ring until abscission checkpoint signaling is terminated at late cytokinesis. AURKB phosphorylates the CPC complex subunits BIRC5/survivin, CDCA8/borealin and INCENP. Phosphorylation of INCENP leads to increased AURKB activity. Other known AURKB substrates involved in centromeric functions and mitosis are CENPA, DES/desmin, GPAF, KIF2C, NSUN2, RACGAP1, SEPTIN1, VIM/vimentin, HASPIN, and histone H3. A positive feedback loop involving HASPIN and AURKB contributes to localization of CPC to centromeres. Phosphorylation of VIM controls vimentin filament segregation in cytokinetic process, whereas histone H3 is phosphorylated at 'Ser-10' and 'Ser-28' during mitosis (H3S10ph and H3S28ph, respectively). AURKB is also required for kinetochore localization of BUB1 and SGO1. Phosphorylation of p53/TP53 negatively regulates its transcriptional activity. Key regulator of active promoters in resting B- and T-lymphocytes: acts by mediating phosphorylation of H3S28ph at active promoters in resting B-cells, inhibiting RNF2/RING1B-mediated ubiquitination of histone H2A and enhancing binding and activity of the USP16 deubiquitinase at transcribed genes. Acts as an inhibitor of CGAS during mitosis: catalyzes phosphorylation of the N-terminus of CGAS during the G2-M transition, blocking CGAS liquid phase separation and activation, and thereby preventing CGAS-induced autoimmunity. Phosphorylates KRT5 during anaphase and telophase. Phosphorylates ATXN10 which promotes phosphorylation of ATXN10 by PLK1 and may play a role in the regulation of cytokinesis and stimulating the proteasomal degradation of ATXN10. |
| Protein Name | Aurora Kinase B Aurora 1 Aurora- And Ipl1-Like Midbody-Associated Protein 1 Aim-1 Aurora/Ipl1-Related Kinase 2 Ark-2 Aurora-Related Kinase 2 Stk-1 Serine/Threonine-Protein Kinase 12 Serine/Threonine-Protein Kinase 5 Serine/Threonine-Protein Kinase Aurora-B |
| Database Links | Reactome: R-HSA-141444 Reactome: R-HSA-174178 Reactome: R-HSA-2467813 Reactome: R-HSA-2500257 Reactome: R-HSA-4615885 Reactome: R-HSA-5663220 Reactome: R-HSA-6804756 Reactome: R-HSA-68877 Reactome: R-HSA-9022692 Reactome: R-HSA-9648025 |
| Cellular Localisation | Nucleus Chromosome Centromere Kinetochore Cytoplasm Cytoskeleton Spindle Midbody Localizes On Chromosome Arms And Inner Centromeres From Prophase Through Metaphase And Then Transferring To The Spindle Midzone And Midbody From Anaphase Through Cytokinesis Colocalized With Gamma Tubulin In The Midbody Proper Localization Of The Active Thr-232-Phosphorylated Form During Metaphase May Be Dependent Upon Interaction With Spdyc Colocalized With Sirt2 During Cytokinesis With The Midbody Localization (And Probably Targeting Of The Cpc) To The Inner Centromere Occurs Predominantly In Regions With Overlapping Mitosis-Specific Histone Phosphorylations H3pt3 And H2apt12 |
| Alternative Antibody Names | Anti-Aurora Kinase B antibody Anti-Aurora 1 antibody Anti-Aurora- And Ipl1-Like Midbody-Associated Protein 1 antibody Anti-Aim-1 antibody Anti-Aurora/Ipl1-Related Kinase 2 antibody Anti-Ark-2 antibody Anti-Aurora-Related Kinase 2 antibody Anti-Stk-1 antibody Anti-Serine/Threonine-Protein Kinase 12 antibody Anti-Serine/Threonine-Protein Kinase 5 antibody Anti-Serine/Threonine-Protein Kinase Aurora-B antibody Anti-AURKB antibody Anti-AIK2 antibody Anti-AIM1 antibody Anti-AIRK2 antibody Anti-ARK2 antibody Anti-STK1 antibody Anti-STK12 antibody Anti-STK5 antibody |
Information sourced from Uniprot.org