• Immunofluorescence analysis of U-2 OS cells using ATXN2 antibody (STJ119084) at dilution of 1:100. Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of L929 cells using ATXN2 antibody (STJ119084) at dilution of 1:100. Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of C6 cells using ATXN2 antibody (STJ119084) at dilution of 1:100. Blue: DAPI for nuclear staining.
  • Immunohistochemistry analysis of paraffin-embedded human colon carcinoma using ATXN2 antibody (STJ119084) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded rat brain using ATXN2 antibody (STJ119084) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of Rat uterus, using ATXN2 Rabbit polyclonal antibody (STJ119084) at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-ATXN2 antibody (1250-1331) (STJ119084)

SKU:
STJ119084

£169.50 - £449.50
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Host: Rabbit
Applications: WB/IHC/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-ATXN2 (1250-1331) is suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IHC-P 1:50-1:200
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: ATXN2
Gene ID: 6311
Uniprot ID: ATX2_HUMAN
Immunogen Region: 1250-1331
Immunogen: A synthetic peptide corresponding to a sequence within amino acids 1250-1331 of human ATXN2 (NP_002964.3).
Immunogen Sequence: HVQSGMVPSHPTAHAPMMLM TTQPPGGPQAALAQSALQPI PVSTTAHFPYMTHPSVQAHH QQQL
Tissue Specificity Expressed in the brain, heart, liver, skeletal muscle, pancreas and placenta. Isoform 1 is predominant in the brain and spinal cord. Isoform 4 is more abundant in the cerebellum. In the brain, broadly expressed in the amygdala, caudate nucleus, corpus callosum, hippocampus, hypothalamus, substantia nigra, subthalamic nucleus and thalamus.
Function Involved in EGFR trafficking, acting as negative regulator of endocytic EGFR internalization at the plasma membrane.
Protein Name Ataxin-2
Spinocerebellar Ataxia Type 2 Protein
Trinucleotide Repeat-Containing Gene 13 Protein
Cellular Localisation Cytoplasm
Alternative Antibody Names Anti-Ataxin-2 antibody
Anti-Spinocerebellar Ataxia Type 2 Protein antibody
Anti-Trinucleotide Repeat-Containing Gene 13 Protein antibody
Anti-ATXN2 antibody
Anti-ATX2 antibody
Anti-SCA2 antibody
Anti-TNRC13 antibody

Information sourced from Uniprot.org

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