| Host: |
Rabbit |
| Applications: |
IHC-P |
| Reactivity: |
Human |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Rabbit monoclonal antibody anti-ATRX (2200-2450aa) is suitable for use in Immunohistochemistry research applications. |
| Clonality: |
Monoclonal |
| Clone ID: |
ZR244 |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
Tris-HCI buffer containing stabilizing protein (BSA) and <0.1% ProClin |
| Purification: |
Affinity purified |
| Dilution Range: |
1:100-200 |
| Storage Instruction: |
Store at 2‐8°C for up to 24 months. Predilute: Ready to use, no reconstitution necessary. Concentrate: Use dilution range and appropriate lab‐standardized diluent. Stability after dilution: 7 days at 24°C, 3 months at 2‐8°C, 6months at ‐20°C. |
| Gene Symbol: |
ATRX |
| Gene ID: |
546 |
| Uniprot ID: |
ATRX_HUMAN |
| Immunogen Region: |
2200-2450aa |
| Specificity: |
Positive control: High-grade glioma |
| Immunogen: |
Recombinant fragment (around aa2200-2450) of human ATRX protein |
| Tissue Specificity | Ubiquitous. |
| Post Translational Modifications | Phosphorylated at serine residues during mitose. Phosphorylation may promote the release from the nuclear matrix and progression to mitosis. |
| Function | Involved in transcriptional regulation and chromatin remodeling. Facilitates DNA replication in multiple cellular environments and is required for efficient replication of a subset of genomic loci. Binds to DNA tandem repeat sequences in both telomeres and euchromatin and in vitro binds DNA quadruplex structures. May help stabilizing G-rich regions into regular chromatin structures by remodeling G4 DNA and incorporating H3.3-containing nucleosomes. Catalytic component of the chromatin remodeling complex ATRX:DAXX which has ATP-dependent DNA translocase activity and catalyzes the replication-independent deposition of histone H3.3 in pericentric DNA repeats outside S-phase and telomeres, and the in vitro remodeling of H3.3-containing nucleosomes. Its heterochromatin targeting is proposed to involve a combinatorial readout of histone H3 modifications (specifically methylation states of H3K9 and H3K4) and association with CBX5. Involved in maintaining telomere structural integrity in embryonic stem cells which probably implies recruitment of CBX5 to telomeres. Reports on the involvement in transcriptional regulation of telomeric repeat-containing RNA (TERRA) are conflicting.according to a report, it is not sufficient to decrease chromatin condensation at telomeres nor to increase expression of telomeric RNA in fibroblasts. May be involved in telomere maintenance via recombination in ALT (alternative lengthening of telomeres) cell lines. Acts as negative regulator of chromatin incorporation of transcriptionally repressive histone MACROH2A1, particularily at telomeres and the alpha-globin cluster in erythroleukemic cells. Participates in the allele-specific gene expression at the imprinted IGF2/H19 gene locus. On the maternal allele, required for the chromatin occupancy of SMC1 and CTCTF within the H19 imprinting control region (ICR) and involved in esatblishment of histone tails modifications in the ICR. May be involved in brain development and facial morphogenesis. Binds to zinc-finger coding genes with atypical chromatin signatures and regulates its H3K9me3 levels. Forms a complex with ZNF274, TRIM28 and SETDB1 to facilitate the deposition and maintenance of H3K9me3 at the 3' exons of zinc-finger genes. |
| Protein Name | Transcriptional Regulator AtrxAtp-Dependent Helicase AtrxX-Linked Helicase IiX-Linked Nuclear ProteinXnpZnf-Hx |
| Database Links | Reactome: R-HSA-9670095Reactome: R-HSA-9670613Reactome: R-HSA-9670615 |
| Cellular Localisation | NucleusChromosomeTelomerePml BodyAssociated With Pericentromeric Heterochromatin During Interphase And MitosisProbably By Interacting With Cbx5/Hp1 AlphaColocalizes With Histone H33DaxxHira And Asf1a At Pml-Nuclear BodiesColocalizes With Cohesin (Smc1 And Smc3) And Mecp2 At The Maternal H19 Icr |
| Alternative Antibody Names | Anti-Transcriptional Regulator Atrx antibodyAnti-Atp-Dependent Helicase Atrx antibodyAnti-X-Linked Helicase Ii antibodyAnti-X-Linked Nuclear Protein antibodyAnti-Xnp antibodyAnti-Znf-Hx antibodyAnti-ATRX antibodyAnti-RAD54L antibodyAnti-XH2 antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
