Anti-Atrogin-1 antibody (N-Term) (STJA0041067)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
ImmunogenA synthetic peptide (coupled to carrier protein) corresponding to amino acids from the N-terminal region of mouse Atrogin. This sequence is highly homologous to human and rat Atrogin (F-box only protein 32).
General Information
| Short Description | Rabbit polyclonal anti-Atrogin-1 (N-Term) for use in ELISA, WB and IHC in Human, Mouse and Rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | ELISA/WB/IHC |
| Host | Rabbit |
| Reactivity | Human/Mouse/Rat |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Purification | Antigen Affinity Purified |
| Dilution Range | WB 1:1000IHC 1:100 |
| Formulation | PBS + 1 mg/ml BSA, 0.05% NaN3 and 50% glycerol |
| Storage Instruction | Storage at-20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at-20°C. |
Target Information
| Gene Symbol | Fbxo32 |
| Gene ID | 67731 |
| Uniprot ID | FBX32_MOUSE |
| Immunogen | A synthetic peptide (coupled to carrier protein) corresponding to amino acids from the N-terminal region of mouse Atrogin. This sequence is highly homologous to human and rat Atrogin (F-box only protein 32). |
| Immunogen Region | N-Term |
| Immunogen Sequence | Mouse |
| Specificity | This antibody detects a 41 kDa* protein corresponding to the apparent molecular mass of Atrogin on SDS-PAGE immunoblots of mouse muscle tissue lysates. Similar results were seen in C2C12 cell lysate and the band observed in western blot is specifical |
Additional Info
| Tissue Specificity | Specifically expressed in cardiac and skeletal muscle. |
| Function | Substrate recognition component of a SCF (SKP1-CUL1-F-box protein) E3 ubiquitin-protein ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins. Probably recognizes and binds to phosphorylated target proteins during skeletal muscle atrophy. Recognizes TERF1. |
| Protein Name | F-Box Only Protein 32Atrogin-1Muscle Atrophy F-Box ProteinMafbx |
| Database Links | Reactome: R-MMU-8951664Reactome: -MMU-983168 |
| Cellular Localisation | CytoplasmNucleusShuttles Between Cytoplasm And The Nucleus |
| Alternative Antibody Names | Anti-F-Box Only Protein 32 antibodyAnti-Atrogin-1 antibodyAnti-Muscle Atrophy F-Box Protein antibodyAnti-Mafbx antibodyAnti-Fbxo32 antibody |
Information sourced from Uniprot.org