• Immunoprecipitation analysis of 200 Mu g extracts of HeLa cells using 1 Mu g ATRIP antibody (STJ116274). Western blot was performed from the immunoprecipitate using ATRIP antibody (STJ116274) at a dilution of 1:1000.
  • Western blot analysis of various lysates using ATRIP Rabbit polyclonal antibody (STJ116274) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Anti-ATRIP antibody (1-260) (STJ116274)

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STJ116274

$151.88 - $705.38
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Host: Rabbit
Applications: WB/IF/IP
Reactivity: Human
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-ATRIP (1-260) is suitable for use in Western Blot, Immunofluorescence and Immunoprecipitation research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IF/ICC 1:50-1:200
IP 1:20-1:50
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: ATRIP
Gene ID: 84126
Uniprot ID: ATRIP_HUMAN
Immunogen Region: 1-260
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-260 of human ATRIP (NP_569055.1).
Immunogen Sequence: MAGTSAPGSKRRSEPPAPRP GPPPGTGHPPSKRARGFSAA AAPDPDDPFGAHGDFTADDL EELDTLASQALSQCPAAARD VSSDHKVHRLLDGMSKNPSG KNRETVPIKDNFELEVLQAQ YKELKEKMKVMEEEVLIKNG EIKILRDSLHQTESVLEEQR RSHFLLEQEKTQALSDKEKE FSKKLQSLQSELQFKDAEMN ELRTKLQTSERANKLAAPSV SHVSPRKNPSVVIKPEACS
Tissue Specificity Ubiquitous.
Post Translational Modifications Phosphorylated by ATR.
Function Required for checkpoint signaling after DNA damage. Required for ATR expression, possibly by stabilizing the protein.
Protein Name Atr-Interacting Protein
Atm And Rad3-Related-Interacting Protein
Database Links Reactome: R-HSA-176187
Reactome: R-HSA-5685938
Reactome: R-HSA-5693607
Reactome: R-HSA-5693616
Reactome: R-HSA-6783310
Reactome: R-HSA-6804756
Reactome: R-HSA-69473
Reactome: R-HSA-9709570
Cellular Localisation Nucleus
Redistributes To Discrete Nuclear Foci Upon Dna Damage
Alternative Antibody Names Anti-Atr-Interacting Protein antibody
Anti-Atm And Rad3-Related-Interacting Protein antibody
Anti-ATRIP antibody
Anti-AGS1 antibody

Information sourced from Uniprot.org

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