Anti-ATP7B antibody (1251-1354) (STJ27643)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
General Information
| Short Description | Rabbit polyclonal ATP7B (1251-1354) antibody for use in WB, IHC-P and ELISA in human, mouse and rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/IHC-P/ELISA |
| Host | Rabbit |
| Reactivity | Human/Mouse/Rat |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | Lot specific |
| Purification | Affinity purification |
| Dilution Range | WB:1:1000-1:2000IHC-P:1:50-1:200ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Formulation | PBS with 0.05% Proclin300, 50% Glycerol, pH 7.3. |
| Storage Instruction | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | ATP7B |
| Gene ID | 540 |
| Uniprot ID | ATP7B_HUMAN |
| Immunogen Region | 1251-1354 |
| Immunogen Sequence | SSVSVVLSSLQLKCYKKPDL ERYEAQAHGHMKPLTASQVS VHIGMDDRWRDSPRATPWDQ VSYVSQVSLSSLTSDKPSRH SAAADDDGDKWSLLLNGRDE E |
| Specificity | Recombinant fusion protein containing a sequence corresponding to amino acids 1251-1351 of human ATP7B (NP_001230111.1). |
Additional Info
| Tissue Specificity | Most abundant in liver and kidney and also found in brain. Isoform 2 is expressed in brain but not in liver. The cleaved form WND/140 kDa is found in liver cell lines and other tissues. |
| Post Translational Modifications | Isoform 1 may be proteolytically cleaved at the N-terminus to produce the WND/140 kDa form. |
| Function | Copper ion transmembrane transporter involved in the export of copper out of the cells. It is involved in copper homeostasis in the liver, where it ensures the efflux of copper from hepatocytes into the bile in response to copper overload. |
| Protein Name | Copper-Transporting Atpase 2Copper Pump 2Wilson Disease-Associated Protein Cleaved Into - Wnd/140 Kda |
| Database Links | Reactome: R-HSA-936837 |
| Cellular Localisation | Golgi ApparatusTrans-Golgi Network MembraneMulti-Pass Membrane ProteinLate EndosomePredominantly Found In The Trans-Golgi Network (Tgn)Localized In The Trans-Golgi Network Under Low Copper ConditionsRedistributes To Cytoplasmic Vesicles When Cells Are Exposed To Elevated Copper LevelsAnd Then Recycles Back To The Trans-Golgi Network When Copper Is RemovedIsoform 1: Golgi Apparatus MembraneIsoform 2: CytoplasmWnd/140 Kda: Mitochondrion |
| Alternative Antibody Names | Anti-Copper-Transporting Atpase 2 antibodyAnti-Copper Pump 2 antibodyAnti-Wilson Disease-Associated Protein Cleaved Into - Wnd/140 Kda antibodyAnti-ATP7B antibodyAnti-PWD antibodyAnti-WC1 antibodyAnti-WND antibody |
Information sourced from Uniprot.org