| Host: | Rabbit |
| Applications: | WB/IHC/IF/ELISA |
| Reactivity: | Human/Mouse |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description : | Rabbit polyclonal antibody anti-V-type proton ATPase subunit H (341-390 aa) is suitable for use in Western Blot, Immunohistochemistry, Immunofluorescence and ELISA research applications. |
| Clonality : | Polyclonal |
| Conjugation: | Unconjugated |
| Isotype: | IgG |
| Formulation: | Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide. |
| Purification: | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Concentration: | 1 mg/mL |
| Dilution Range: | WB 1:500-1:2000 IHC 1:100-1:300 ELISA 1:20000 IF 1:50-200 |
| Storage Instruction: | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: | ATP6V1H |
| Gene ID: | 51606 |
| Uniprot ID: | VATH_HUMAN |
| Immunogen Region: | 341-390 aa |
| Specificity: | V-ATPase H Polyclonal Antibody detects endogenous levels of V-ATPase H protein. |
| Immunogen: | The antiserum was produced against synthesized peptide derived from the human ATP6V1H at the amino acid range 341-390 |
| Function | Subunit of the V1 complex of vacuolar(H+)-ATPase (V-ATPase), a multisubunit enzyme composed of a peripheral complex (V1) that hydrolyzes ATP and a membrane integral complex (V0) that translocates protons. V-ATPase is responsible for acidifying and maintaining the pH of intracellular compartments and in some cell types, is targeted to the plasma membrane, where it is responsible for acidifying the extracellular environment. Subunit H is essential for V-ATPase activity, but not for the assembly of the complex. Involved in the endocytosis mediated by clathrin-coated pits, required for the formation of endosomes. |
| Protein Name | V-Type Proton Atpase Subunit H V-Atpase Subunit H Nef-Binding Protein 1 Nbp1 Protein Vma13 Homolog V-Atpase 50/57 Kda Subunits Vacuolar Proton Pump Subunit H Vacuolar Proton Pump Subunit Sfd |
| Database Links | Reactome: R-HSA-1222556 Reactome: R-HSA-167590 Reactome: R-HSA-182218 Reactome: R-HSA-77387 Reactome: R-HSA-917977 Reactome: R-HSA-9636467 Reactome: R-HSA-9639288 Reactome: R-HSA-983712 Reactome: R-HSA-9857377 |
| Cellular Localisation | Cytoplasmic Vesicle Clathrin-Coated Vesicle Membrane Peripheral Membrane Protein |
| Alternative Antibody Names | Anti-V-Type Proton Atpase Subunit H antibody Anti-V-Atpase Subunit H antibody Anti-Nef-Binding Protein 1 antibody Anti-Nbp1 antibody Anti-Protein Vma13 Homolog antibody Anti-V-Atpase 50/57 Kda Subunits antibody Anti-Vacuolar Proton Pump Subunit H antibody Anti-Vacuolar Proton Pump Subunit Sfd antibody Anti-ATP6V1H antibody Anti-CGI-11 antibody |
Information sourced from Uniprot.org