• Immunohistochemistry analysis of ATP6V1G3 in paraffin-embedded mouse kidney using ATP6V1G3 Rabbit polyclonal antibody (STJ116653) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of lysates from mouse kidney, using ATP6V1G3 Rabbit polyclonal antibody (STJ116653) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-ATP6V1G3 antibody (1-118) (STJ116653)

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STJ116653

£169.50 - £449.50
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Host: Rabbit
Applications: WB/IHC
Reactivity: Mouse
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-ATP6V1G3 (1-118) is suitable for use in Western Blot and Immunohistochemistry research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IHC-P 1:50-1:100
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: ATP6V1G3
Gene ID: 127124
Uniprot ID: VATG3_HUMAN
Immunogen Region: 1-118
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-118 of human ATP6V1G3 (NP_573569.1).
Immunogen Sequence: MTSQSQGIHQLLQAEKRAKD KLEEAKKRKGKRLKQAKEEA MVEIDQYRMQRDKEFRLKQS KIMGSQNNLSDEIEEQTLGK IQELNGHYNKYMESVMNQLL SMVCDMKPEIHVNYRATN
Tissue Specificity Kidney.
Function Subunit of the V1 complex of vacuolar(H+)-ATPase (V-ATPase), a multisubunit enzyme composed of a peripheral complex (V1) that hydrolyzes ATP and a membrane integral complex (V0) that translocates protons. V-ATPase is responsible for acidifying and maintaining the pH of intracellular compartments and in some cell types, is targeted to the plasma membrane, where it is responsible for acidifying the extracellular environment.
Protein Name V-Type Proton Atpase Subunit G 3
V-Atpase Subunit G 3
V-Atpase 13 Kda Subunit 3
Vacuolar Proton Pump Subunit G 3
Database Links Reactome: R-HSA-1222556
Reactome: R-HSA-77387
Reactome: R-HSA-917977
Reactome: R-HSA-9639288
Reactome: R-HSA-983712
Alternative Antibody Names Anti-V-Type Proton Atpase Subunit G 3 antibody
Anti-V-Atpase Subunit G 3 antibody
Anti-V-Atpase 13 Kda Subunit 3 antibody
Anti-Vacuolar Proton Pump Subunit G 3 antibody
Anti-ATP6V1G3 antibody
Anti-ATP6G3 antibody

Information sourced from Uniprot.org

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