• Western blot analysis of 1, mouse-lung 2, mouse-brain 3, mouse-spleen 4, mouse-kidney cells using primary antibody diluted at 1:500 (4°C overnight). Secondary antibody：Goat Anti-rabbit IgG IRDye 800 ( diluted at 1:5000, 25°C, 1 hour)

Anti-ATP6AP1 antibody (421-470 aa) (STJ99321)

SKU:
STJ99321

£46.00 - £226.00
Current Stock:
Host: Rabbit
Applications: WB/ELISA
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-V-type proton ATPase subunit S1 (421-470 aa) is suitable for use in Western Blot and ELISA research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide.
Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration: 1 mg/mL
Dilution Range: WB 1:500-2000
ELISA 1:10000-20000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: ATP6AP1
Gene ID: 537
Uniprot ID: VAS1_HUMAN
Immunogen Region: 421-470 aa
Specificity: V-ATPase S1 Polyclonal Antibody detects endogenous levels of V-ATPase S1
Immunogen: Synthesized peptide derived from the human V-ATPase S1 at the amino acid range 421-470
Post Translational Modifications N-glycosylated.
Function Accessory subunit of the proton-transporting vacuolar (V)-ATPase protein pump, which is required for luminal acidification of secretory vesicles. Guides the V-type ATPase into specialized subcellular compartments, such as neuroendocrine regulated secretory vesicles or the ruffled border of the osteoclast, thereby regulating its activity. Involved in membrane trafficking and Ca(2+)-dependent membrane fusion. May play a role in the assembly of the V-type ATPase complex (Probable). In aerobic conditions, involved in intracellular iron homeostasis, thus triggering the activity of Fe(2+) prolyl hydroxylase (PHD) enzymes, and leading to HIF1A hydroxylation and subsequent proteasomal degradation. In islets of Langerhans cells, may regulate the acidification of dense-core secretory granules.
Protein Name V-Type Proton Atpase Subunit S1
V-Atpase Subunit S1
Protein Xap-3
V-Atpase Ac45 Subunit
V-Atpase S1 Accessory Protein
Vacuolar Proton Pump Subunit S1
Database Links Reactome: R-HSA-77387
Reactome: R-HSA-8980692
Reactome: R-HSA-917977
Reactome: R-HSA-983712
Cellular Localisation Endoplasmic Reticulum Membrane
Single-Pass Type I Membrane Protein
Endoplasmic Reticulum-Golgi Intermediate Compartment Membrane
Cytoplasmic Vesicle
Secretory Vesicle
Synaptic Vesicle Membrane
Clathrin-Coated Vesicle Membrane
Not Detected In Trans-Golgi Network
Alternative Antibody Names Anti-V-Type Proton Atpase Subunit S1 antibody
Anti-V-Atpase Subunit S1 antibody
Anti-Protein Xap-3 antibody
Anti-V-Atpase Ac45 Subunit antibody
Anti-V-Atpase S1 Accessory Protein antibody
Anti-Vacuolar Proton Pump Subunit S1 antibody
Anti-ATP6AP1 antibody
Anti-ATP6IP1 antibody
Anti-ATP6S1 antibody
Anti-VATPS1 antibody
Anti-XAP3 antibody

Information sourced from Uniprot.org

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