• Immunohistochemical analysis of paraffin-embedded Human thyroid gland. Antibody was diluted at 1:100 (4°C overnight). High-pressure and temperature Tris-EDTA, pH8.0 was used for antigen retrieval. Negetive contrl (right) obtaned from antibody was pre-absorbed by immunogen peptide.
  • Western blot analysis of lysates from HeLa cells and Jurkat cells, using ATPG Antibody. The lane on the right is blocked with the synthesized peptide.
  • Western blot analysis of various cells using ATP5C1 Polyclonal Antibody

Anti-ATP5F1C antibody (131-180 aa) (STJ91765)

SKU:
STJ91765

Current Stock:
Host: Rabbit
Applications: WB/IHC/IF/ELISA
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-ATP synthase subunit gamma, mitochondrial (131-180 aa) is suitable for use in Western Blot, Immunohistochemistry, Immunofluorescence and ELISA research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide.
Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration: 1 mg/mL
Dilution Range: WB 1:500-1:2000
IHC 1:100-1:300
ELISA 1:40000
IF 1:50-200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: ATP5F1C
Gene ID: 509
Uniprot ID: ATPG_HUMAN
Immunogen Region: 131-180 aa
Specificity: ATP5C1 Polyclonal Antibody detects endogenous levels of ATP5C1 protein.
Immunogen: The antiserum was produced against synthesized peptide derived from the human ATP5C1 at the amino acid range 131-180
Function Mitochondrial membrane ATP synthase (F(1)F(0) ATP synthase or Complex V) produces ATP from ADP in the presence of a proton gradient across the membrane which is generated by electron transport complexes of the respiratory chain. F-type ATPases consist of two structural domains, F(1) - containing the extramembraneous catalytic core, and F(0) - containing the membrane proton channel, linked together by a central stalk and a peripheral stalk. During catalysis, ATP synthesis in the catalytic domain of F(1) is coupled via a rotary mechanism of the central stalk subunits to proton translocation. Part of the complex F(1) domain and the central stalk which is part of the complex rotary element. The gamma subunit protrudes into the catalytic domain formed of alpha(3)beta(3). Rotation of the central stalk against the surrounding alpha(3)beta(3) subunits leads to hydrolysis of ATP in three separate catalytic sites on the beta subunits.
Protein Name Atp Synthase Subunit Gamma - Mitochondrial
Atp Synthase F1 Subunit Gamma
F-Atpase Gamma Subunit
Database Links Reactome: R-HSA-163210
Reactome: R-HSA-8949613
Cellular Localisation Mitochondrion Inner Membrane
Peripheral Membrane Protein
Matrix Side
Alternative Antibody Names Anti-Atp Synthase Subunit Gamma - Mitochondrial antibody
Anti-Atp Synthase F1 Subunit Gamma antibody
Anti-F-Atpase Gamma Subunit antibody
Anti-ATP5F1C antibody
Anti-ATP5C antibody
Anti-ATP5C1 antibody
Anti-ATP5CL1 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance