• Immunohistochemistry analysis of paraffin-embedded mouse stomach using ATP4B Rabbit monoclonal antibody (STJ11103011) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded rat stomach using ATP4B Rabbit monoclonal antibody (STJ11103011) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9. 0 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of extracts of Mouse stomach, using ATP4B antibody (STJ11103011) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.
  • Western blot analysis of extracts of various cell lines, using ATP4B antibody (STJ11103011) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.

Anti-ATP4B antibody (192-291) [S1MR] (STJ11103011)

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STJ11103011

£212.50 - £501.50
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Host: Rabbit
Applications: WB/IHC
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit monoclonal antibody anti-ATP4B (192-291) is suitable for use in Western Blot and Immunohistochemistry research applications.
Clonality: Monoclonal
Clone ID: S1MR
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 0.05% BSA, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
IHC-P 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: ATP4B
Gene ID: 496
Uniprot ID: ATP4B_HUMAN
Immunogen Region: 192-291
Immunogen: A synthetic peptide corresponding to a sequence within amino acids 192-291 of human ATP4B (P51164).
Immunogen Sequence: SNGSAPRVDCAFLDQPRELG QPLQVKYYPPNGTFSLHYFP YYGKKAQPHYSNPLVAAKLL NIPRNAEVAIVCKVMAEHVT FNNPHDPYEGKVEFKLKIEK
Post Translational Modifications N-glycosylation is necessary for assembly and functional expression of the pump at the plasma membrane.
Function The beta subunit of the gastric H(+)/K(+) ATPase pump which transports H(+) ions in exchange for K(+) ions across the apical membrane of parietal cells. Plays a structural and regulatory role in the assembly and membrane targeting of a functionally active pump. Within a transport cycle, the transfer of a H(+) ion across the membrane is coupled to ATP hydrolysis and is associated with a transient phosphorylation of the alpha subunit that shifts the pump conformation from inward-facing (E1) to outward-facing state (E2). Interacts with the phosphorylation domain of the alpha subunit and functions as a ratchet, stabilizing the lumenal-open E2 conformation and preventing the reverse reaction of the transport cycle.
Protein Name Potassium-Transporting Atpase Subunit Beta
Gastric H(+/K(+ Atpase Subunit Beta
Proton Pump Beta Chain
Database Links Reactome: R-HSA-936837
Cellular Localisation Apical Cell Membrane
Single-Pass Type Ii Membrane Protein
Cell Membrane
Localized In The Apical Canalicular Membrane Of Parietal Cells
Alternative Antibody Names Anti-Potassium-Transporting Atpase Subunit Beta antibody
Anti-Gastric H(+/K(+ Atpase Subunit Beta antibody
Anti-Proton Pump Beta Chain antibody
Anti-ATP4B antibody

Information sourced from Uniprot.org

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