Anti-ATP4B antibody (160-240 aa) (STJ191197)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
ImmunogenSynthesized peptide derived from the human protein at the amino acid range 160-240
General Information
| Short Description | Rabbit polyclonal anti-Potassium-transporting ATPase subunit beta/K+ ATPase subunit beta (160-240 aa) for use in WB and ELISA in Human, Rat and Mouse samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/ELISA |
| Host | Rabbit |
| Reactivity | Human/Rat/Mouse |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | 1 mg/mL |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Dilution Range | WB 1:500-2000ELISA 1:5000-20000 |
| Formulation | Liquid in PBS containing 50% Glycerol and 0.02% Sodium Azide. |
| Storage Instruction | Store at-20ยฐC for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | ATP4B |
| Gene ID | 496 |
| Uniprot ID | ATP4B_HUMAN |
| Immunogen | Synthesized peptide derived from the human protein at the amino acid range 160-240 |
| Immunogen Region | 160-240 aa |
| Specificity | ATP4B Polyclonal Antibody detects endogenous levels of protein. |
Additional Info
| Function | The beta subunit of the gastric H(+)/K(+) ATPase pump which transports H(+) ions in exchange for K(+) ions across the apical membrane of parietal cells. Plays a structural and regulatory role in the assembly and membrane targeting of a functionally active pump. Within a transport cycle, the transfer of a H(+) ion across the membrane is coupled to ATP hydrolysis and is associated with a transient phosphorylation of the alpha subunit that shifts the pump conformation from inward-facing (E1) to outward-facing state (E2). Interacts with the phosphorylation domain of the alpha subunit and functions as a ratchet, stabilizing the lumenal-open E2 conformation and preventing the reverse reaction of the transport cycle. |
| Protein Name | Potassium-Transporting Atpase Subunit BetaGastric H(+/K(+ Atpase Subunit BetaProton Pump Beta Chain |
| Database Links | Reactome: R-HSA-936837 |
| Cellular Localisation | Apical Cell MembraneSingle-Pass Type Ii Membrane ProteinCell MembraneLocalized In The Apical Canalicular Membrane Of Parietal Cells |
| Alternative Antibody Names | Anti-Potassium-Transporting Atpase Subunit Beta antibodyAnti-Gastric H(+/K(+ Atpase Subunit Beta antibodyAnti-Proton Pump Beta Chain antibodyAnti-ATP4B antibody |
Information sourced from Uniprot.org