Anti-ATP2B1 antibody (312-327) (STJ72685)
SPECIFICATIONS
ClonalityPolyclonal
HostGoat
ConjugationUnconjugated
IsotypeIgG
General Information
| Short Description | Goat polyclonal antibody anti-ATP2B1 (312-327) is suitable for use in ELISA and Western Blot research applications. |
| Applications | Pep-ELISA/WB |
| Host | Goat |
| Reactivity | Human/Mouse/Rat/Dog/Pig/Cow |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | 0.5 mg/mL |
| Purification | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Dilution Range | Peptide ELISA: antibody detection limit dilution 1:64000.WB: Approx 150kDa band observed in lysates of cell line HeLa (calculated MW of 135kDa according to NP_001673.2). Recommended concentration: 0.03-0.1µg/ml. |
| Formulation | 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. NA |
| Storage Instruction | Store at-20°C on receipt and minimise freeze-thaw cycles. |
Target Information
| Gene Symbol | ATP2B1 |
| Gene ID | 490 |
| Uniprot ID | AT2B1_HUMAN |
| Accession Number | NP_001001323.1; NP_001673.2 |
| Immunogen Region | 312-327 |
| Immunogen Sequence | KQDGAIENRNKAKAQD |
| Specificity | This antibody is expected to recognize both reported isoforms (NP_001001323.1; NP_001673.2). |
Additional Info
| Function | Catalyzes the hydrolysis of ATP coupled with the transport of calcium from the cytoplasm to the extracellular space thereby maintaining intracellular calcium homeostasis. Plays a role in blood pressure regulation through regulation of intracellular calcium concentration and nitric oxide production leading to regulation of vascular smooth muscle cells vasoconstriction. Positively regulates bone mineralization through absorption of calcium from the intestine. Plays dual roles in osteoclast differentiation and survival by regulating RANKL-induced calcium oscillations in preosteoclasts and mediating calcium extrusion in mature osteoclasts. Regulates insulin sensitivity through calcium/calmodulin signaling pathway by regulating AKT1 activation and NOS3 activation in endothelial cells. May play a role in synaptic transmission by modulating calcium and proton dynamics at the synaptic vesicles. |
| Protein Name | Plasma Membrane Calcium-Transporting Atpase 1Plasma Membrane Calcium Atpase Isoform 1Pmca1Plasma Membrane Calcium Pump Isoform 1 |
| Database Links | Reactome: R-HSA-418359Reactome: R-HSA-5578775Reactome: R-HSA-936837Reactome: R-HSA-9662360 |
| Cellular Localisation | Cell MembraneMulti-Pass Membrane ProteinBasolateral Cell MembraneSynapsePresynaptic Cell MembraneCytoplasmic VesicleSecretory VesicleSynaptic Vesicle MembraneColocalizes With Sv2a In Photoreceptor Synaptic TerminalsColocalizes With Nptn To The Immunological SynapseColocalizes With Epb41 To The Basolateral Membrane In EnterocytePreferentially Sorted To Recycling Synaptic Vesicles |
| Alternative Antibody Names | Anti-Plasma Membrane Calcium-Transporting Atpase 1 antibodyAnti-Plasma Membrane Calcium Atpase Isoform 1 antibodyAnti-Pmca1 antibodyAnti-Plasma Membrane Calcium Pump Isoform 1 antibodyAnti-ATP2B1 antibodyAnti-PMCA1 antibody |
Information sourced from Uniprot.org