| Host: |
Rabbit |
| Applications: |
WB/IHC/IF/ELISA |
| Reactivity: |
Human/Mouse/Rat/Chicken |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Rabbit polyclonal antibody anti-Sarcoplasmic/endoplasmic reticulum calcium ATPase 2-ATPase 2 ATPase (841-890 aa) is suitable for use in Western Blot, Immunohistochemistry, Immunofluorescence and ELISA research applications. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide. |
| Purification: |
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Concentration: |
1 mg/mL |
| Dilution Range: |
WB 1:500-1:2000IHC-P 1:100-1:300ELISA 1:20000IF 1:50-200 |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: |
ATP2A2 |
| Gene ID: |
488 |
| Uniprot ID: |
AT2A2_HUMAN |
| Immunogen Region: |
841-890 aa |
| Specificity: |
SERCA2 Polyclonal Antibody detects endogenous levels of SERCA2 protein. |
| Immunogen: |
The antiserum was produced against synthesized peptide derived from the C-terminal region of human ATP2A2 at the amino acid range 841-890 |
| Post Translational Modifications | Nitrated under oxidative stress. Nitration on the two tyrosine residues inhibits catalytic activity. Serotonylated on Gln residues by TGM2 in response to hypoxia, leading to its inactivation. |
| Function | This magnesium-dependent enzyme catalyzes the hydrolysis of ATP coupled with the translocation of calcium from the cytosol to the sarcoplasmic reticulum lumen. Involved in autophagy in response to starvation. Upon interaction with VMP1 and activation, controls ER-isolation membrane contacts for autophagosome formation. Also modulates ER contacts with lipid droplets, mitochondria and endosomes. In coordination with FLVCR2 mediates heme-stimulated switching from mitochondrial ATP synthesis to thermogenesis. Isoform 2: Involved in the regulation of the contraction/relaxation cycle. Acts as a regulator of TNFSF11-mediated Ca(2+) signaling pathways via its interaction with TMEM64 which is critical for the TNFSF11-induced CREB1 activation and mitochondrial ROS generation necessary for proper osteoclast generation. Association between TMEM64 and SERCA2 in the ER leads to cytosolic Ca(2+) spiking for activation of NFATC1 and production of mitochondrial ROS, thereby triggering Ca(2+) signaling cascades that promote osteoclast differentiation and activation. |
| Protein Name | Sarcoplasmic/Endoplasmic Reticulum Calcium Atpase 2Serca2Sr Ca(2+-Atpase 2Calcium Pump 2Calcium-Transporting Atpase Sarcoplasmic Reticulum Type - Slow Twitch Skeletal Muscle IsoformEndoplasmic Reticulum Class 1/2 Ca(2+ Atpase |
| Database Links | Reactome: R-HSA-1912420Reactome: R-HSA-418359Reactome: R-HSA-5578775Reactome: R-HSA-936837 |
| Cellular Localisation | Endoplasmic Reticulum MembraneMulti-Pass Membrane ProteinSarcoplasmic Reticulum MembraneColocalizes With Flvcr2 At The Mitochondrial-Er Contact Junction |
| Alternative Antibody Names | Anti-Sarcoplasmic/Endoplasmic Reticulum Calcium Atpase 2 antibodyAnti-Serca2 antibodyAnti-Sr Ca(2+-Atpase 2 antibodyAnti-Calcium Pump 2 antibodyAnti-Calcium-Transporting Atpase Sarcoplasmic Reticulum Type - Slow Twitch Skeletal Muscle Isoform antibodyAnti-Endoplasmic Reticulum Class 1/2 Ca(2+ Atpase antibodyAnti-ATP2A2 antibodyAnti-ATP2B antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
