• Immunohistochemistry analysis of SERCA2/ATP2A2 in paraffin-embedded mouse heart using SERCA2/ATP2A2 Rabbit polyclonal antibody (STJ113534) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of SERCA2/ATP2A2 in paraffin-embedded mouse lung using SERCA2/ATP2A2 Rabbit polyclonal antibody (STJ113534) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of SERCA2/ATP2A2 in paraffin-embedded rat heart using SERCA2/ATP2A2 Rabbit polyclonal antibody (STJ113534) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of lysates from Mouse heart, using SERCA2/ATP2A2 Rabbit polyclonal antibody (STJ113534) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.

Anti-ATP2A2 antibody (111-253) (STJ113534)

SKU:
STJ113534

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Host: Rabbit
Applications: WB/IHC
Reactivity: Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-ATP2A2/SERCA2 (111-253) is suitable for use in Western Blot and Immunohistochemistry research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IHC-P 1:50-1:100
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: ATP2A2
Gene ID: 488
Uniprot ID: AT2A2_HUMAN
Immunogen Region: 111-253
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 111-253 of human SERCA2/ATP2A2 (NP_733765.1).
Immunogen Sequence: NAENAIEALKEYEPEMGKVY RQDRKSVQRIKAKDIVPGDI VEIAVGDKVPADIRLTSIKS TTLRVDQSILTGESVSVIKH TDPVPDPRAVNQDKKNMLFS GTNIAAGKAMGVVVATGVNT EIGKIRDEMVATEQERTPLQ QKL
Tissue Specificity Isoform 1 is widely expressed in smooth muscle and nonmuscle tissues such as in adult skin epidermis, with highest expression in liver, pancreas and lung, and intermediate expression in brain, kidney and placenta. Also expressed at lower levels in heart and skeletal muscle. Isoforms 2 and 3 are highly expressed in the heart and slow twitch skeletal muscle. Expression of isoform 3 is predominantly restricted to cardiomyocytes and in close proximity to the sarcolemma. Both isoforms are mildly expressed in lung, kidney, liver, pancreas and placenta. Expression of isoform 3 is amplified during monocytic differentiation and also observed in the fetal heart.
Post Translational Modifications Nitrated under oxidative stress. Nitration on the two tyrosine residues inhibits catalytic activity. Serotonylated on Gln residues by TGM2 in response to hypoxia, leading to its inactivation.
Function This magnesium-dependent enzyme catalyzes the hydrolysis of ATP coupled with the translocation of calcium from the cytosol to the sarcoplasmic reticulum lumen. Involved in autophagy in response to starvation. Upon interaction with VMP1 and activation, controls ER-isolation membrane contacts for autophagosome formation. Also modulates ER contacts with lipid droplets, mitochondria and endosomes. In coordination with FLVCR2 mediates heme-stimulated switching from mitochondrial ATP synthesis to thermogenesis. Isoform 2: Involved in the regulation of the contraction/relaxation cycle. Acts as a regulator of TNFSF11-mediated Ca(2+) signaling pathways via its interaction with TMEM64 which is critical for the TNFSF11-induced CREB1 activation and mitochondrial ROS generation necessary for proper osteoclast generation. Association between TMEM64 and SERCA2 in the ER leads to cytosolic Ca(2+) spiking for activation of NFATC1 and production of mitochondrial ROS, thereby triggering Ca(2+) signaling cascades that promote osteoclast differentiation and activation.
Protein Name Sarcoplasmic/Endoplasmic Reticulum Calcium Atpase 2
Serca2
Sr Ca(2+-Atpase 2
Calcium Pump 2
Calcium-Transporting Atpase Sarcoplasmic Reticulum Type - Slow Twitch Skeletal Muscle Isoform
Endoplasmic Reticulum Class 1/2 Ca(2+ Atpase
Database Links Reactome: R-HSA-1912420
Reactome: R-HSA-418359
Reactome: R-HSA-5578775
Reactome: R-HSA-936837
Cellular Localisation Endoplasmic Reticulum Membrane
Multi-Pass Membrane Protein
Sarcoplasmic Reticulum Membrane
Colocalizes With Flvcr2 At The Mitochondrial-Er Contact Junction
Alternative Antibody Names Anti-Sarcoplasmic/Endoplasmic Reticulum Calcium Atpase 2 antibody
Anti-Serca2 antibody
Anti-Sr Ca(2+-Atpase 2 antibody
Anti-Calcium Pump 2 antibody
Anti-Calcium-Transporting Atpase Sarcoplasmic Reticulum Type - Slow Twitch Skeletal Muscle Isoform antibody
Anti-Endoplasmic Reticulum Class 1/2 Ca(2+ Atpase antibody
Anti-ATP2A2 antibody
Anti-ATP2B antibody

Information sourced from Uniprot.org

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