• Immunohistochemistry analysis of paraffin-embedded mouse kidney using ATP1B1 Rabbit monoclonal antibody (STJ11102258) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded rat kidney using ATP1B1 Rabbit monoclonal antibody (STJ11102258) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of extracts of U2OS cells, using ATP1B1 Rabbit monoclonal antibody (STJ11102258) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 30s.
  • Western blot analysis of extracts of various cell lines, using ATP1B1 Rabbit monoclonal antibody (STJ11102258) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-ATP1B1 antibody (204-303) [S8MR] (STJ11102258)

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STJ11102258

£212.50 - £501.50
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Host: Rabbit
Applications: WB/IHC
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit monoclonal antibody anti-ATP1B1 (204-303) is suitable for use in Western Blot and Immunohistochemistry research applications.
Clonality: Monoclonal
Clone ID: S8MR
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 0.05% BSA, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IHC-P 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: ATP1B1
Gene ID: 481
Uniprot ID: AT1B1_HUMAN
Immunogen Region: 204-303
Immunogen: A synthetic peptide corresponding to a sequence within amino acids 204-303 of human ATP1B1 (P05026).
Immunogen Sequence: YNPNVLPVQCTGKRDEDKDK VGNVEYFGLGNSPGFPLQYY PYYGKLLQPKYLQPLLAVQF TNLTMDTEIRIECKAYGENI GYSEKDRFQGRFDVKIEVKS
Tissue Specificity Found in most tissues.
Post Translational Modifications Glutathionylated. N-glycosylated.
Function This is the non-catalytic component of the active enzyme, which catalyzes the hydrolysis of ATP coupled with the exchange of Na(+) and K(+) ions across the plasma membrane. The beta subunit regulates, through assembly of alpha/beta heterodimers, the number of sodium pumps transported to the plasma membrane. Plays a role in innate immunity by enhancing virus-triggered induction of interferons (IFNs) and interferon stimulated genes (ISGs). Mechanistically, enhances the ubiquitination of TRAF3 and TRAF6 as well as the phosphorylation of TAK1 and TBK1. Involved in cell adhesion and establishing epithelial cell polarity.
Protein Name Sodium/Potassium-Transporting Atpase Subunit Beta-1
Sodium/Potassium-Dependent Atpase Subunit Beta-1
Database Links Reactome: R-HSA-210991
Reactome: R-HSA-5578775
Reactome: R-HSA-936837
Reactome: R-HSA-9679191
Cellular Localisation Cell Membrane
Single-Pass Type Ii Membrane Protein
Apical Cell Membrane
Sarcolemma
Colocalizes With Obscn At The Intercalated Disk And Sarcolemma In Cardiomyocytes
Localizes In Long Striations At The Level Of Z And M Lines
Alternative Antibody Names Anti-Sodium/Potassium-Transporting Atpase Subunit Beta-1 antibody
Anti-Sodium/Potassium-Dependent Atpase Subunit Beta-1 antibody
Anti-ATP1B1 antibody
Anti-ATP1B antibody

Information sourced from Uniprot.org

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