Anti-ATGL/Desnutrin antibody (Internal) (STJ71118)
SPECIFICATIONS
ClonalityPolyclonal
HostGoat
ConjugationUnconjugated
IsotypeIgG
General Information
| Short Description | Goat polyclonal antibody anti-ATGL/Desnutrin (Internal) is suitable for use in ELISA, Western Blot and Immunohistochemistry research applications. |
| Applications | Pep-ELISA/WB/IHC |
| Host | Goat |
| Reactivity | Human/Mouse/Rat/Dog/Pig/Cow |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | 0.5 mg/mL |
| Purification | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Dilution Range | Peptide ELISA: antibody detection limit dilution 1:32000.WB: Approx 60kDa band observed in Human Adipose lysates (calculated MW of 55.3kDa according to NP_065109.1). Recommended concentration: 0.5-2µg/ml. Primary incubation was 1 hour. Prelimina |
| Formulation | 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. NA |
| Storage Instruction | Store at-20°C on receipt and minimise freeze-thaw cycles. |
Target Information
| Gene Symbol | PNPLA2 |
| Gene ID | 57104 |
| Uniprot ID | PLPL2_HUMAN |
| Accession Number | NP_065109.1 |
| Immunogen Region | Internal |
| Immunogen Sequence | NIIEVSKEARKR |
| Specificity | Pease note this antibody was designed using the Mouse sequence NP_080078.1, which differs by 2 amino acids from the Human sequence NP_065109.1. |
Additional Info
| Post Translational Modifications | Phosphorylation at Ser-404 by PKA is increased during fasting and moderate intensity exercise, and moderately increases lipolytic activity. Phosphorylation at Ser-404 is increased upon beta-adrenergic stimulation. Ubiquitinated by PEX2 in response to reactive oxygen species (ROS), leading to its degradation. Ubiquitination is stimulated by LDAH. |
| Function | Catalyzes the initial step in triglyceride hydrolysis in adipocyte and non-adipocyte lipid droplets. Exhibits a strong preference for the hydrolysis of long-chain fatty acid esters at the sn-2 position of the glycerol backbone and acts coordinately with LIPE/HLS and DGAT2 within the lipolytic cascade. Also possesses acylglycerol transacylase and phospholipase A2 activities. Transfers fatty acid from triglyceride to retinol, hydrolyzes retinylesters, and generates 1,3-diacylglycerol from triglycerides. Regulates adiposome size and may be involved in the degradation of adiposomes. Catalyzes the formation of an ester bond between hydroxy fatty acids and fatty acids derived from triglycerides or diglycerides to generate fatty acid esters of hydroxy fatty acids (FAHFAs) in adipocytes. Acts antagonistically with LDAH in regulation of cellular lipid stores. Inhibits LDAH-stimulated lipid droplet fusion. May play an important role in energy homeostasis. May play a role in the response of the organism to starvation, enhancing hydrolysis of triglycerides and providing free fatty acids to other tissues to be oxidized in situations of energy depletion. |
| Protein Name | Patatin-Like Phospholipase Domain-Containing Protein 2Adipose Triglyceride LipaseCalcium-Independent Phospholipase A2-ZetaIpla2-ZetaDesnutrinPigment Epithelium-Derived Factor ReceptorPedf-RTts2.2Transport-Secretion Protein 2Tts2 |
| Database Links | Reactome: R-HSA-1482883Reactome: R-HSA-381426Reactome: R-HSA-8957275 |
| Cellular Localisation | Lipid DropletCell MembraneMulti-Pass Membrane ProteinCytoplasm |
| Alternative Antibody Names | Anti-Patatin-Like Phospholipase Domain-Containing Protein 2 antibodyAnti-Adipose Triglyceride Lipase antibodyAnti-Calcium-Independent Phospholipase A2-Zeta antibodyAnti-Ipla2-Zeta antibodyAnti-Desnutrin antibodyAnti-Pigment Epithelium-Derived Factor Receptor antibodyAnti-Pedf-R antibodyAnti-Tts2.2 antibodyAnti-Transport-Secretion Protein 2 antibodyAnti-Tts2 antibodyAnti-PNPLA2 antibodyAnti-ATGL antibodyAnti-FP17548 antibody |
Information sourced from Uniprot.org