Anti-ATG9A antibody (STJ98589)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
ImmunogenSynthetic Peptide of Atg9a
General Information
| Short Description | Rabbit polyclonal anti-Autophagy-related protein 9A for use in WB and ELISA in Human, Mouse and Rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/ELISA |
| Host | Rabbit |
| Reactivity | Human/Mouse/Rat |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | 1 mg/mL |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Dilution Range | WB 1:500-2000ELISA 1:10000-20000 |
| Formulation | Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide. |
| Storage Instruction | Store at-20ยฐC for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | ATG9A |
| Gene ID | 79065 |
| Uniprot ID | ATG9A_HUMAN |
| Immunogen | Synthetic Peptide of Atg9a |
| Specificity | Atg9a Polyclonal Antibody detects endogenous levels of Atg9a |
Additional Info
| Function | Phospholipid scramblase involved in autophagy by mediating autophagosomal membrane expansion. Cycles between the preautophagosomal structure/phagophore assembly site (PAS) and the cytoplasmic vesicle pool and supplies membrane for the growing autophagosome. Lipid scramblase activity plays a key role in preautophagosomal structure/phagophore assembly by distributing the phospholipids that arrive through ATG2 (ATG2A or ATG2B) from the cytoplasmic to the luminal leaflet of the bilayer, thereby driving autophagosomal membrane expansion. Also required to supply phosphatidylinositol 4-phosphate to the autophagosome initiation site by recruiting the phosphatidylinositol 4-kinase beta (PI4KB) in a process dependent on ARFIP2, but not ARFIP1. In addition to autophagy, also plays a role in necrotic cell death. |
| Protein Name | Autophagy-Related Protein 9aApg9-Like 1Matg9 |
| Database Links | Reactome: R-HSA-1632852Reactome: R-HSA-5205685 |
| Cellular Localisation | Preautophagosomal Structure MembraneMulti-Pass Membrane ProteinCytoplasmic VesicleAutophagosome MembraneGolgi ApparatusTrans-Golgi Network MembraneLate Endosome MembraneRecycling Endosome MembraneEndoplasmic Reticulum MembraneMitochondrion MembraneMainly Localizes To The Trans-Golgi Network (Tgn) And The Endosomal SystemCycles Between Them Though Vesicle TraffickingExport From The Tgn To Promote Formation Of Autophagosomes Is Mediated By The Ap-4 ComplexUnder Amino Acid Starvation Or Rapamycin TreatmentRedistributes To Preautophagosomal Structure/Phagophore Assembly Site (Pas)The Starvation-Induced Redistribution Depends On Ulk1Atg13As Well As Sh3glb1Upon Autophagy InductionA Small Portion Transiently Localizes To The Autophagic MembranesRecruited To Damaged Mitochondria During Mitophagy In A Rimoc1-Dependent Manner |
| Alternative Antibody Names | Anti-Autophagy-Related Protein 9a antibodyAnti-Apg9-Like 1 antibodyAnti-Matg9 antibodyAnti-ATG9A antibodyAnti-APG9L1 antibody |
Information sourced from Uniprot.org