| Host: |
Rabbit |
| Applications: |
WB/IHC-P/IP/ELISA |
| Reactivity: |
Human/Mouse/Rat |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Clonality: |
Monoclonal |
| Clone ID: |
S4MR |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
PBS with 0.02% Sodium Azide, 0.05% BSA, 50% Glycerol, pH 7.3. |
| Purification: |
Affinity purification |
| Concentration: |
Lot specific |
| Dilution Range: |
WB:1:1000-1:6000IHC-P:1:200-1:800IP:0.5 Mu g-4 Mu g antibody for 200 Mu g-400 Mu g extracts of whole cellsELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirement |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: |
ATG7 |
| Gene ID: |
10533 |
| Uniprot ID: |
ATG7_HUMAN |
| Immunogen Region: |
1-100 |
| Specificity: |
A synthetic peptide corresponding to a sequence within amino acids 1-100 of human Atg7 (Apg7) (O95352). |
| Immunogen Sequence: |
MAAATGDPGLSKLQFAPFSS ALDVGFWHELTQKKLNEYRL DEAPKDIKGYYYNGDSAGLP ARLTLEFSAFDMSAPTPARC CPAIGTLYNTNTLESFKTAD |
| Tissue Specificity | Widely expressed, especially in kidney, liver, lymph nodes and bone marrow. |
| Post Translational Modifications | Acetylated by EP300. Polyubiquitinated on Lys-45 via 'Lys-63'-linked ubiquitin by TRIM32.this modification positiely regulates ATG8 and ATG12 activating enzyme activity leading to initiation of autophagy under metabolic stress. |
| Function | E1-like activating enzyme involved in the 2 ubiquitin-like systems required for cytoplasm to vacuole transport (Cvt) and autophagy. Activates ATG12 for its conjugation with ATG5 as well as the ATG8 family proteins for their conjugation with phosphatidylethanolamine. Both systems are needed for the ATG8 association to Cvt vesicles and autophagosomes membranes. Required for autophagic death induced by caspase-8 inhibition. Facilitates LC3-I lipidation with phosphatidylethanolamine to form LC3-II which is found on autophagosomal membranes. Required for mitophagy which contributes to regulate mitochondrial quantity and quality by eliminating the mitochondria to a basal level to fulfill cellular energy requirements and preventing excess ROS production. Modulates p53/TP53 activity to regulate cell cycle and survival during metabolic stress. Also plays a key role in the maintenance of axonal homeostasis, the prevention of axonal degeneration, the maintenance of hematopoietic stem cells, the formation of Paneth cell granules, as well as in adipose differentiation. Plays a role in regulating the liver clock and glucose metabolism by mediating the autophagic degradation of CRY1 (clock repressor) in a time-dependent manner. |
| Protein Name | Ubiquitin-Like Modifier-Activating Enzyme Atg7Atg12-Activating Enzyme E1 Atg7Autophagy-Related Protein 7Apg7-LikeHagp7Ubiquitin-Activating Enzyme E1-Like Protein |
| Database Links | Reactome: R-HSA-1632852Reactome: R-HSA-6798695Reactome: R-HSA-6802952Reactome: R-HSA-983168 |
| Cellular Localisation | CytoplasmPreautophagosomal StructureLocalizes Also To Discrete Punctae Along The Ciliary Axoneme And To The Base Of The Ciliary Axoneme |
| Alternative Antibody Names | Anti-Ubiquitin-Like Modifier-Activating Enzyme Atg7 antibodyAnti-Atg12-Activating Enzyme E1 Atg7 antibodyAnti-Autophagy-Related Protein 7 antibodyAnti-Apg7-Like antibodyAnti-Hagp7 antibodyAnti-Ubiquitin-Activating Enzyme E1-Like Protein antibodyAnti-ATG7 antibodyAnti-APG7L antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
