![Immunoprecipitation analysis of 300 Mu g extracts from 293T cells using 3 Mu g [KO Validated] ATG5 Rabbit monoclonal antibody (STJ11101759). Western blot was performed from the immunoprecipitate using [KO Validated] ATG5 Rabbit monoclonal antibody (STJ11101759) at a dilution of 1:1000.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/124x110/products/90674/360687/STJ11101759_1__34437.1713125386.jpg?c=1 "Immunoprecipitation analysis of 300 Mu g extracts from 293T cells using 3 Mu g [KO Validated] ATG5 Rabbit monoclonal antibody (STJ11101759). Western blot was performed from the immunoprecipitate using [KO Validated] ATG5 Rabbit monoclonal antibody (STJ11101759) at a dilution of 1:1000.")
![Immunohistochemistry analysis of ATG5 in paraffin-embedded human placenta using [KO Validated] ATG5 Rabbit monoclonal antibody (STJ11101759) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/124x110/products/90674/360688/STJ11101759_2__68354.1713125386.jpg?c=1 "Immunohistochemistry analysis of ATG5 in paraffin-embedded human placenta using [KO Validated] ATG5 Rabbit monoclonal antibody (STJ11101759) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.")
![Western blot analysis of various lysates using [KO Validated] ATG5 Rabbit monoclonal antibody (STJ11101759) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/124x110/products/90674/360689/STJ11101759_3__26957.1713125387.jpg?c=1 "Western blot analysis of various lysates using [KO Validated] ATG5 Rabbit monoclonal antibody (STJ11101759) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.")
![Western blot analysis of lysates from wild type (WT) and ATG5 knockout (KO) 293T cells, using [KO Validated] ATG5 Rabbit monoclonal antibody (STJ11101759) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/124x110/products/90674/360690/STJ11101759_4__02238.1713125388.jpg?c=1 "Western blot analysis of lysates from wild type (WT) and ATG5 knockout (KO) 293T cells, using [KO Validated] ATG5 Rabbit monoclonal antibody (STJ11101759) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.")
![Immunoprecipitation analysis of 300 Mu g extracts from 293T cells using 3 Mu g [KO Validated] ATG5 Rabbit monoclonal antibody (STJ11101759). Western blot was performed from the immunoprecipitate using [KO Validated] ATG5 Rabbit monoclonal antibody (STJ11101759) at a dilution of 1:1000.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/1280x1280/products/90674/360687/STJ11101759_1__34437.1713125386.jpg?c=1 "Immunoprecipitation analysis of 300 Mu g extracts from 293T cells using 3 Mu g [KO Validated] ATG5 Rabbit monoclonal antibody (STJ11101759). Western blot was performed from the immunoprecipitate using [KO Validated] ATG5 Rabbit monoclonal antibody (STJ11101759) at a dilution of 1:1000.")
Anti-ATG5 antibody (1-100) [S9MR] (STJ11101759)
SPECIFICATIONS
ClonalityMonoclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
General Information
| Short Description | Rabbit monoclonal ATG5 (1-100) antibody for use in WB, IHC-P, IP and ELISA in human, mouse and rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/IHC-P/IP/ELISA |
| Host | Rabbit |
| Reactivity | Human/Mouse/Rat |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Monoclonal |
| Clone ID | S9MR |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | Lot specific |
| Purification | Affinity purification |
| Dilution Range | WB:1:1000-1:6000IHC-P:1:200-1:2000IP:0.5 Mu g-4 Mu g antibody for 200 Mu g-400 Mu g extracts of whole cellsELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requiremen |
| Formulation | PBS with 0.09% Sodium Azide, 0.05% BSA, 50% Glycerol, pH7.3. |
| Storage Instruction | Store at-20ยฐC for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | ATG5 |
| Gene ID | 9474 |
| Uniprot ID | ATG5_HUMAN |
| Immunogen Region | 1-100 |
| Immunogen Sequence | MTDDKDVLRDVWFGRIPTCF TLYQDEITEREAEPYYLLLP RVSYLTLVTDKVKKHFQKVM RQEDISEIWFEYEGTPLKWH YPIGLLFDLLASSSALPWNI |
| Specificity | A synthetic peptide corresponding to a sequence within amino acids 1-100 of human ATG5 (Q9H1Y0). |
Additional Info
| Tissue Specificity | Ubiquitous. The mRNA is present at similar levels in viable and apoptotic cells, whereas the protein is dramatically highly expressed in apoptotic cells. |
| Post Translational Modifications | Conjugated to ATG12.which is essential for autophagy, but is not required for association with isolation membrane. Acetylated by EP300. |
| Function | Involved in autophagic vesicle formation. Conjugation with ATG12, through a ubiquitin-like conjugating system involving ATG7 as an E1-like activating enzyme and ATG10 as an E2-like conjugating enzyme, is essential for its function. The ATG12-ATG5 conjugate acts as an E3-like enzyme which is required for lipidation of ATG8 family proteins and their association to the vesicle membranes. Involved in mitochondrial quality control after oxidative damage, and in subsequent cellular longevity. Plays a critical role in multiple aspects of lymphocyte development and is essential for both B and T lymphocyte survival and proliferation. Required for optimal processing and presentation of antigens for MHC II. Involved in the maintenance of axon morphology and membrane structures, as well as in normal adipocyte differentiation. Promotes primary ciliogenesis through removal of OFD1 from centriolar satellites and degradation of IFT20 via the autophagic pathway. As part of the ATG8 conjugation system with ATG12 and ATG16L1, required for recruitment of LRRK2 to stressed lysosomes and induction of LRRK2 kinase activity in response to lysosomal stress. May play an important role in the apoptotic process, possibly within the modified cytoskeleton. Its expression is a relatively late event in the apoptotic process, occurring downstream of caspase activity. Plays a crucial role in IFN-gamma-induced autophagic cell death by interacting with FADD. (Microbial infection) May act as a proviral factor. In association with ATG12, negatively regulates the innate antiviral immune response by impairing the type I IFN production pathway upon vesicular stomatitis virus (VSV) infection. Required for the translation of incoming hepatitis C virus (HCV) RNA and, thereby, for initiation of HCV replication, but not required once infection is established. |
| Protein Name | Autophagy Protein 5Apg5-LikeApoptosis-Specific Protein |
| Database Links | Reactome: R-HSA-1632852Reactome: R-HSA-5205685Reactome: R-HSA-8934903Reactome: R-HSA-936440 |
| Cellular Localisation | CytoplasmPreautophagosomal Structure MembranePeripheral Membrane ProteinColocalizes With Nonmuscle ActinThe Conjugate Detaches From The Membrane Immediately Before Or After Autophagosome Formation Is CompletedAlso Localizes To Discrete Punctae Along The Ciliary Axoneme And To The Base Of The Ciliary AxonemeUnder Starved ConditionsThe Atg12-Atg5-Atg16l1 Complex Is Translocated To Phagophores Driven By Rab33b |
| Alternative Antibody Names | Anti-Autophagy Protein 5 antibodyAnti-Apg5-Like antibodyAnti-Apoptosis-Specific Protein antibodyAnti-ATG5 antibodyAnti-APG5L antibodyAnti-ASP antibody |
Information sourced from Uniprot.org