Anti-ATG4C antibody [R08-8G9] (STJA0034051)
SPECIFICATIONS
ClonalityMonoclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
ImmunogenRecombinant protein of human ATG4C
General Information
| Short Description | Rabbit monoclonal anti-ATG4C for use in WB and IHC-P in Human samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/IHC-P |
| Host | Rabbit |
| Reactivity | Human |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Monoclonal |
| Clone ID | R08-8G9 |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | 0.3 mg/mL |
| Purification | Affinity Purified |
| Dilution Range | WB 1:500-1:1000IHC 1:50-1:100 |
| Formulation | 50mM Tris-Glycine (pH 7.4) , 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA |
| Storage Instruction | Store at 4°C short term. Aliquot and store at-20°C long term. Avoid freeze/thaw cycles. |
Target Information
| Gene Symbol | ATG4C |
| Gene ID | 84938 |
| Uniprot ID | ATG4C_HUMAN |
| Immunogen | Recombinant protein of human ATG4C |
Additional Info
| Function | Cysteine protease that plays a key role in autophagy by mediating both proteolytic activation and delipidation of ATG8 family proteins. The protease activity is required for proteolytic activation of ATG8 family proteins: cleaves the C-terminal amino acid of ATG8 proteins MAP1LC3 and GABARAPL2, to reveal a C-terminal glycine. Exposure of the glycine at the C-terminus is essential for ATG8 proteins conjugation to phosphatidylethanolamine (PE) and insertion to membranes, which is necessary for autophagy. In addition to the protease activity, also mediates delipidation of ATG8 family proteins. Catalyzes delipidation of PE-conjugated forms of ATG8 proteins during macroautophagy. Compared to ATG4B, the major protein for proteolytic activation of ATG8 proteins, shows weaker ability to cleave the C-terminal amino acid of ATG8 proteins, while it displays stronger delipidation activity. In contrast to other members of the family, weakly or not involved in phagophore growth during mitophagy. |
| Protein Name | Cysteine Protease Atg4cAut-Like 3 Cysteine EndopeptidaseAutophagy-Related Cysteine Endopeptidase 3Autophagin-3Autophagy-Related Protein 4 Homolog CHsapg4c |
| Database Links | Reactome: R-HSA-1632852 |
| Cellular Localisation | Cytoplasm |
| Alternative Antibody Names | Anti-Cysteine Protease Atg4c antibodyAnti-Aut-Like 3 Cysteine Endopeptidase antibodyAnti-Autophagy-Related Cysteine Endopeptidase 3 antibodyAnti-Autophagin-3 antibodyAnti-Autophagy-Related Protein 4 Homolog C antibodyAnti-Hsapg4c antibodyAnti-ATG4C antibodyAnti-APG4C antibodyAnti-AUTL1 antibodyAnti-AUTL3 antibody |
Information sourced from Uniprot.org