• Western blot analysis of extracts of various cell lines, using ATG2B antibody (STJ110796) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 60s.
  • Western blot analysis of extracts of various cell lines, using ATG2B antibody (STJ110796) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 60s.

Anti-ATG2B antibody (1360-1600) (STJ110796)

SKU:
STJ110796

£169.50 - £449.50
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Host: Rabbit
Applications: WB
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-ATG2B (1360-1600) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: ATG2B
Gene ID: 55102
Uniprot ID: ATG2B_HUMAN
Immunogen Region: 1360-1600
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1360-1600 of human ATG2B (NP_060506.5).
Immunogen Sequence: IQYIASYGDLQTPNKADMKP GAFQRRSKVDSSGRSSSRGP VLPEADQQMLRDLMSDAMEE IDMQQGTSSVKPQANGVLDE KSQIQEPCCSDLFLFPDESG NVSQESGPTYASFSHHFISD AMTGVPTENDDFCILFAPKA AMQEKEEEPVIKIMVDDAIV IRDNYFSLPVNKTDTSKAPL HFPIPVIRYVVKEVSLVWHL YGGKDFGTVPPTSPAKSYIS PHSSPSHTPTRHGRNTVCG
Function Lipid transfer protein required for both autophagosome formation and regulation of lipid droplet morphology and dispersion. Tethers the edge of the isolation membrane (IM) to the endoplasmic reticulum (ER) and mediates direct lipid transfer from ER to IM for IM expansion. Binds to the ER exit site (ERES), which is the membrane source for autophagosome formation, and extracts phospholipids from the membrane source and transfers them to ATG9 (ATG9A or ATG9B) to the IM for membrane expansion. Lipid transfer activity is enhanced by WDR45/WIPI4, which promotes ATG2B-association with phosphatidylinositol 3-monophosphate (PI3P)-containing membranes.
Protein Name Autophagy-Related Protein 2 Homolog B
Cellular Localisation Preautophagosomal Structure Membrane
Peripheral Membrane Protein
Lipid Droplet
Endoplasmic Reticulum Membrane
Alternative Antibody Names Anti-Autophagy-Related Protein 2 Homolog B antibody
Anti-ATG2B antibody
Anti-C14orf103 antibody

Information sourced from Uniprot.org

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