• Immunohistochemistry of rat tissue with Anti-ATG16L1 primary antibody (STJ500180) at 1:50 dilution in Buffer. Section was treated with DAB and Haematoxylin stain. Magnification is at 5X.

Anti-ATG16L1 antibody (560-610) (STJ500180)

SKU:
STJ500180-100

£441.50
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Host: Rabbit
Applications: ELISA/IHC/IP/WB
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-ATG16L1 (560-610) is suitable for use in ELISA, Immunohistochemistry, Immunoprecipitation and Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Purification: Affinity Purified
Concentration: 0.5 µg/µl
Dilution Range: WB: 1:500
ELISA: 1:10, 000
IP: 1:150
IHC: 1:50-1:150
Storage Instruction: Store at-20°C for long term storage. Avoid freeze-thaw cycles.
Gene Symbol: ATG16L1
Gene ID: 55054
Uniprot ID: A16L1_HUMAN
Immunogen Region: 560-610
Immunogen: Synthetic peptide taken within amino acid region 560-610 on human Autophagy-related protein 16-1.
Post Translational Modifications Proteolytic cleavage by activated CASP3 leads to degradation and may regulate autophagy upon cellular stress and apoptotic stimuli. Phosphorylation at Ser-139 promotes association with the ATG12-ATG5 conjugate to form the ATG12-ATG5-ATG16L1 complex.
Function Plays an essential role in both canonical and non-canonical autophagy: interacts with ATG12-ATG5 to mediate the lipidation to ATG8 family proteins (MAP1LC3A, MAP1LC3B, MAP1LC3C, GABARAPL1, GABARAPL2 and GABARAP). Acts as a molecular hub, coordinating autophagy pathways via distinct domains that support either canonical or non-canonical signaling. During canonical autophagy, interacts with ATG12-ATG5 to mediate the conjugation of phosphatidylethanolamine (PE) to ATG8 proteins, to produce a membrane-bound activated form of ATG8. Thereby, controls the elongation of the nascent autophagosomal membrane. Also involved in non-canonical autophagy, a parallel pathway involving conjugation of ATG8 proteins to single membranes at endolysosomal compartments, probably by catalyzing conjugation of phosphatidylserine (PS) to ATG8. Non-canonical autophagy plays a key role in epithelial cells to limit lethal infection by influenza A (IAV) virus. Regulates mitochondrial antiviral signaling (MAVS)-dependent type I interferon (IFN-I) production. Negatively regulates NOD1- and NOD2-driven inflammatory cytokine response. Instead, promotes an autophagy-dependent antibacterial pathway together with NOD1 or NOD2. Plays a role in regulating morphology and function of Paneth cell.
Protein Name Autophagy-Related Protein 16-1
Apg16-Like 1
Database Links Reactome: R-HSA-1632852
Cellular Localisation Cytoplasm
Preautophagosomal Structure Membrane
Peripheral Membrane Protein
Endosome Membrane
Lysosome Membrane
Recruited To Omegasomes Membranes By Wipi2
Omegasomes Are Endoplasmic Reticulum Connected Strutures At The Origin Of Preautophagosomal Structures
Localized To Preautophagosomal Structure (Pas) Where It Is Involved In The Membrane Targeting Of Atg5
Localizes Also To Discrete Punctae Along The Ciliary Axoneme
Upon Activation Of Non-Canonical Autophagy
Recruited To Single-Membrane Endolysosomal Compartments
Alternative Antibody Names Anti-Autophagy-Related Protein 16-1 antibody
Anti-Apg16-Like 1 antibody
Anti-ATG16L1 antibody
Anti-APG16L antibody
Anti-UNQ9393 antibody
Anti-PRO34307 antibody

Information sourced from Uniprot.org

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