• Immunofluorescence analysis of U2OS cells using ATG14 Rabbit polyclonal antibody (STJ29664) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
  • Western blot analysis of extracts of Neuro-2a cells, using ATG14 antibody (STJ29664) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 180s.
  • Western blot analysis of extracts of various cell lines, using ATG14 antibody (STJ29664) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.

Anti-ATG14 antibody (340-440) (STJ29664)

SKU:
STJ29664

£169.50 - £449.50
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Host: Rabbit
Applications: WB/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-ATG14 (340-440) is suitable for use in Western Blot and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: ATG14
Gene ID: 22863
Uniprot ID: BAKOR_HUMAN
Immunogen Region: 340-440
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 340-440 of human ATG14 (NP_055739.2).
Immunogen Sequence: SKQKFTRAVKKLNANILYLC FSQHVNLDQLQPLHTLRNLM YLVSPSSEHLGRSGPFEVRA DLEESMEFVDPGVAGESDES GDERVSDEETDLGTDWENLP S
Function Required for both basal and inducible autophagy. Determines the localization of the autophagy-specific PI3-kinase complex PI3KC3-C1. Plays a role in autophagosome formation and MAP1LC3/LC3 conjugation to phosphatidylethanolamine. Promotes BECN1 translocation from the trans-Golgi network to autophagosomes. Enhances PIK3C3 activity in a BECN1-dependent manner. Essential for the autophagy-dependent phosphorylation of BECN1. Stimulates the phosphorylation of BECN1, but suppresses the phosphorylation PIK3C3 by AMPK. Binds to STX17-SNAP29 binary t-SNARE complex on autophagosomes and primes it for VAMP8 interaction to promote autophagosome-endolysosome fusion. Modulates the hepatic lipid metabolism.
Protein Name Beclin 1-Associated Autophagy-Related Key Regulator
Barkor
Autophagy-Related Protein 14-Like Protein
Atg14l
Database Links Reactome: R-HSA-1632852
Reactome: R-HSA-9705671
Reactome: R-HSA-983170
Cellular Localisation Cytoplasm
Endoplasmic Reticulum Membrane
Peripheral Membrane Protein
Preautophagosomal Structure Membrane
Cytoplasmic Vesicle
Autophagosome Membrane
Cytosolic Under Nutrient-Rich Conditions
Following Autophagy Stimuli
Such As Starvation Or Rapamycin Induction
Predominantly Detected In Cytoplasmic Foci
Identified As Isolation Membranes And Autophagosomes
Accumulates On Highly Curved Ptdins(3)P Enriched Autophagic Membrane Via Its Bats Domain To Sense And Maintain Membrane Curvature
Localizes Also To Discrete Punctae Along The Ciliary Axoneme And To The Base Of The Ciliary Axoneme
Alternative Antibody Names Anti-Beclin 1-Associated Autophagy-Related Key Regulator antibody
Anti-Barkor antibody
Anti-Autophagy-Related Protein 14-Like Protein antibody
Anti-Atg14l antibody
Anti-ATG14 antibody
Anti-ATG14L antibody
Anti-KIAA0831 antibody

Information sourced from Uniprot.org

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