| Host: |
Rabbit |
| Applications: |
WB/IF/ICC/ELISA |
| Reactivity: |
Human/Mouse/Rat |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
PBS with 0.02% Sodium Azide, 50% Glycerol, pH 7.3. |
| Purification: |
Affinity purification |
| Concentration: |
Lot specific |
| Dilution Range: |
WB:1:500-1:1000IF/ICC:1:50-1:200ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: |
ATG14 |
| Gene ID: |
22863 |
| Uniprot ID: |
BAKOR_HUMAN |
| Immunogen Region: |
340-440 |
| Specificity: |
Recombinant fusion protein containing a sequence corresponding to amino acids 340-440 of human ATG14 (NP_055739.2). |
| Immunogen Sequence: |
SKQKFTRAVKKLNANILYLC FSQHVNLDQLQPLHTLRNLM YLVSPSSEHLGRSGPFEVRA DLEESMEFVDPGVAGESDES GDERVSDEETDLGTDWENLP S |
| Post Translational Modifications | Ubiquitinated via 'Lys-6', 'Lys-11' and 'Lys-63'-linked polyubiquitin chains on multiple lysines by MARCHF7, leading to ATG14 aggregation and loss of interaction with STX17. |
| Function | Required for both basal and inducible autophagy. Determines the localization of the autophagy-specific PI3-kinase complex PI3KC3-C1. Plays a role in autophagosome formation and MAP1LC3/LC3 conjugation to phosphatidylethanolamine. Promotes BECN1 translocation from the trans-Golgi network to autophagosomes. Enhances PIK3C3 activity in a BECN1-dependent manner. Essential for the autophagy-dependent phosphorylation of BECN1. Stimulates the phosphorylation of BECN1, but suppresses the phosphorylation PIK3C3 by AMPK. Binds to STX17-SNAP29 binary t-SNARE complex on autophagosomes and primes it for VAMP8 interaction to promote autophagosome-endolysosome fusion. Modulates the hepatic lipid metabolism. |
| Protein Name | Beclin 1-Associated Autophagy-Related Key RegulatorBarkorAutophagy-Related Protein 14-Like ProteinAtg14l |
| Database Links | Reactome: R-HSA-1632852Reactome: R-HSA-9705671Reactome: R-HSA-983170 |
| Cellular Localisation | CytoplasmEndoplasmic Reticulum MembranePeripheral Membrane ProteinPreautophagosomal Structure MembraneCytoplasmic VesicleAutophagosome MembraneCytosolic Under Nutrient-Rich ConditionsFollowing Autophagy StimuliSuch As Starvation Or Rapamycin InductionPredominantly Detected In Cytoplasmic FociIdentified As Isolation Membranes And AutophagosomesAccumulates On Highly Curved Ptdins(3)P Enriched Autophagic Membrane Via Its Bats Domain To Sense And Maintain Membrane CurvatureLocalizes Also To Discrete Punctae Along The Ciliary Axoneme And To The Base Of The Ciliary Axoneme |
| Alternative Antibody Names | Anti-Beclin 1-Associated Autophagy-Related Key Regulator antibodyAnti-Barkor antibodyAnti-Autophagy-Related Protein 14-Like Protein antibodyAnti-Atg14l antibodyAnti-ATG14 antibodyAnti-ATG14L antibodyAnti-KIAA0831 antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
