Anti-ATG13 antibody [R08-8E8] (STJA0033808)
SPECIFICATIONS
ClonalityMonoclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
ImmunogenRecombinant protein of human KIAA0652
General Information
| Short Description | Rabbit monoclonal anti-ATG13 for use in WB and IP in Human, Mouse and Rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/IP |
| Host | Rabbit |
| Reactivity | Human/Mouse/Rat |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Monoclonal |
| Clone ID | R08-8E8 |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | 0.3 mg/mL |
| Purification | Affinity Purified |
| Dilution Range | WB 1:500-1:1000IP 1:20 |
| Formulation | 50mM Tris-Glycine (pH 7.4) , 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA |
| Storage Instruction | Store at 4°C short term. Aliquot and store at-20°C long term. Avoid freeze/thaw cycles. |
Target Information
| Gene Symbol | ATG13 |
| Gene ID | 9776 |
| Uniprot ID | ATG13_HUMAN |
| Immunogen | Recombinant protein of human KIAA0652 |
Additional Info
| Post Translational Modifications | Phosphorylated by ULK1, ULK2 and mTOR. Phosphorylation status depends on nutrient-rich conditions.dephosphorylated during starvation or following treatment with rapamycin. ULK1-mediated phosphorylation of ATG13 at Ser-355 is required for efficient clearance of depolarized mitochondria. |
| Function | Autophagy factor required for autophagosome formation and mitophagy. Target of the TOR kinase signaling pathway that regulates autophagy through the control of the phosphorylation status of ATG13 and ULK1, and the regulation of the ATG13-ULK1-RB1CC1 complex. Through its regulation of ULK1 activity, plays a role in the regulation of the kinase activity of mTORC1 and cell proliferation. |
| Protein Name | Autophagy-Related Protein 13 |
| Database Links | Reactome: R-HSA-1632852 |
| Cellular Localisation | CytoplasmCytosolPreautophagosomal StructureUnder Starvation ConditionsIs Localized To Puncate Structures Primarily Representing The Isolation MembraneThe Isolation Membrane Sequesters A Portion Of The Cytoplasm Resulting In Autophagosome Formation |
| Alternative Antibody Names | Anti-Autophagy-Related Protein 13 antibodyAnti-ATG13 antibodyAnti-KIAA0652 antibody |
Information sourced from Uniprot.org