Anti-ATG13 antibody (281-480 aa) [SRM5259] (STJ11107161)
SPECIFICATIONS
ClonalityMonoclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
General Information
| Short Description | Rabbit monoclonal ATG13 (281-480 aa) antibody for use in WB and ELISA in human, mouse and rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/ELISA |
| Host | Rabbit |
| Reactivity | Human/Mouse/Rat |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Monoclonal |
| Clone ID | SRM5259 |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | Lot specific |
| Purification | Affinity purification |
| Dilution Range | WB:1:500-1:1000ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Formulation | PBS with 0.05% Proclin300, 0.05% BSA, 50% Glycerol, pH 7.3. |
| Storage Instruction | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | ATG13 |
| Gene ID | 9776 |
| Uniprot ID | ATG13_HUMAN |
| Immunogen Region | 281-480 aa |
| Immunogen Sequence | ADLAYPVVFAAGLNATHPHQ LMVPGKEGGVPLAPNQPVHG TQADQERLATCTPSDRTHCA ATPSSSEDTETVSNSSEGRA SPHDVLETIFVRKVGAFVNK PINQVTLTSLDIPFAMFAPK NLELEDTDPMVNPPDSPETE SPLQGSLHSDGSSGGSSGNT HDDFVMIDFKPAFSKDDILP MDLGTFYREFQNPPQLSSLS |
| Specificity | Recombinant fusion protein containing a sequence corresponding to amino acids 281-480 of human ATG13 (NP_001136145.1). |
Additional Info
| Post Translational Modifications | Phosphorylated by ULK1, ULK2 and mTOR. Phosphorylation status depends on nutrient-rich conditions.dephosphorylated during starvation or following treatment with rapamycin. ULK1-mediated phosphorylation of ATG13 at Ser-355 is required for efficient clearance of depolarized mitochondria. |
| Function | Autophagy factor required for autophagosome formation and mitophagy. Target of the TOR kinase signaling pathway that regulates autophagy through the control of the phosphorylation status of ATG13 and ULK1, and the regulation of the ATG13-ULK1-RB1CC1 complex. Through its regulation of ULK1 activity, plays a role in the regulation of the kinase activity of mTORC1 and cell proliferation. |
| Protein Name | Autophagy-Related Protein 13 |
| Database Links | Reactome: R-HSA-1632852 |
| Cellular Localisation | CytoplasmCytosolPreautophagosomal StructureUnder Starvation ConditionsIs Localized To Puncate Structures Primarily Representing The Isolation MembraneThe Isolation Membrane Sequesters A Portion Of The Cytoplasm Resulting In Autophagosome Formation |
| Alternative Antibody Names | Anti-Autophagy-Related Protein 13 antibodyAnti-ATG13 antibodyAnti-KIAA0652 antibody |
Information sourced from Uniprot.org