| Host: |
Rabbit |
| Applications: |
WB |
| Reactivity: |
Human/Mouse/Rat |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Rabbit polyclonal antibody anti-Ubiquitin-like protein ATG12 (80-130 aa) is suitable for use in Western Blot research applications. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide. |
| Purification: |
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Concentration: |
1 mg/mL |
| Dilution Range: |
WB 1:500-2000 |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: |
ATG12 |
| Gene ID: |
9140 |
| Uniprot ID: |
ATG12_HUMAN |
| Immunogen Region: |
80-130 aa |
| Specificity: |
This antibody detects endogenous levels of ATG12 at Human/Mouse/Rat |
| Immunogen: |
Synthesized peptide derived from the human ATG12 at the amino acid range 80-130 |
| Post Translational Modifications | Acetylated by EP300. |
| Function | Ubiquitin-like protein involved in autophagy vesicles formation. Conjugation with ATG5 through a ubiquitin-like conjugating system involving also ATG7 as an E1-like activating enzyme and ATG10 as an E2-like conjugating enzyme, is essential for its function. The ATG12-ATG5 conjugate acts as an E3-like enzyme which is required for lipidation of ATG8 family proteins and their association to the vesicle membranes. (Microbial infection) May act as a proviral factor. In association with ATG5, negatively regulates the innate antiviral immune response by impairing the type I IFN production pathway upon vesicular stomatitis virus (VSV) infection. Required for the translation of incoming hepatitis C virus (HCV) RNA and, thereby, for the initiation of HCV replication, but not required once infection is established. |
| Protein Name | Ubiquitin-Like Protein Atg12Autophagy-Related Protein 12Apg12-Like |
| Database Links | Reactome: R-HSA-1632852Reactome: R-HSA-5205685Reactome: R-HSA-8934903Reactome: R-HSA-936440 |
| Cellular Localisation | CytoplasmPreautophagosomal Structure MembranePeripheral Membrane ProteinTecpr1 Recruits The Atg12-Atg5 Conjugate To The Autolysosomal Membrane |
| Alternative Antibody Names | Anti-Ubiquitin-Like Protein Atg12 antibodyAnti-Autophagy-Related Protein 12 antibodyAnti-Apg12-Like antibodyAnti-ATG12 antibodyAnti-APG12 antibodyAnti-APG12L antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
