• Western blot analysis of lysates from HpeG2 cells, primary antibody was diluted at 1:1000, 4°C over night

Anti-ATG12 antibody (80-130 aa) (STJ195672)

SKU:
STJ195672

Current Stock:
Host: Rabbit
Applications: WB
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-Ubiquitin-like protein ATG12 (80-130 aa) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide.
Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration: 1 mg/mL
Dilution Range: WB 1:500-2000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: ATG12
Gene ID: 9140
Uniprot ID: ATG12_HUMAN
Immunogen Region: 80-130 aa
Specificity: This antibody detects endogenous levels of ATG12 at Human/Mouse/Rat
Immunogen: Synthesized peptide derived from the human ATG12 at the amino acid range 80-130
Post Translational Modifications Acetylated by EP300.
Function Ubiquitin-like protein involved in autophagy vesicles formation. Conjugation with ATG5 through a ubiquitin-like conjugating system involving also ATG7 as an E1-like activating enzyme and ATG10 as an E2-like conjugating enzyme, is essential for its function. The ATG12-ATG5 conjugate acts as an E3-like enzyme which is required for lipidation of ATG8 family proteins and their association to the vesicle membranes. (Microbial infection) May act as a proviral factor. In association with ATG5, negatively regulates the innate antiviral immune response by impairing the type I IFN production pathway upon vesicular stomatitis virus (VSV) infection. Required for the translation of incoming hepatitis C virus (HCV) RNA and, thereby, for the initiation of HCV replication, but not required once infection is established.
Protein Name Ubiquitin-Like Protein Atg12
Autophagy-Related Protein 12
Apg12-Like
Database Links Reactome: R-HSA-1632852
Reactome: R-HSA-5205685
Reactome: R-HSA-8934903
Reactome: R-HSA-936440
Cellular Localisation Cytoplasm
Preautophagosomal Structure Membrane
Peripheral Membrane Protein
Tecpr1 Recruits The Atg12-Atg5 Conjugate To The Autolysosomal Membrane
Alternative Antibody Names Anti-Ubiquitin-Like Protein Atg12 antibody
Anti-Autophagy-Related Protein 12 antibody
Anti-Apg12-Like antibody
Anti-ATG12 antibody
Anti-APG12 antibody
Anti-APG12L antibody

Information sourced from Uniprot.org

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