Anti-ATG12 antibody (STJ13100043)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
ImmunogenA synthetic peptide from mouse ATG12 conjugated to blue carrier protein has been used as the antigen.
General Information
| Short Description | Rabbit polyclonal anti-ATG12 for use in IHC and WB in Rat and Mouse samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | IHC/WB |
| Host | Rabbit |
| Reactivity | Rat/Mouse |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Purification | IgG purified |
| Dilution Range | IHC, WB. Use at a dilution of 1:1:500. The optimal dilution should be determined by the end user. Not yet tested in other applications. For WB, kidney lysate should be prepared in lysing buffer containing 1% Triton-X100, 1% SDS (but no SDC). |
| Formulation | Lyophilised |
| Storage Instruction | Maintain the lyophilised/reconstituted antibodies frozen at-20°C for long term storage and refrigerated at 2-8°C for a shorter term. When reconstituting, Glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles. |
Target Information
| Gene Symbol | Atg12 |
| Gene ID | 67526 |
| Uniprot ID | ATG12_MOUSE |
| Immunogen | A synthetic peptide from mouse ATG12 conjugated to blue carrier protein has been used as the antigen. |
| Specificity | Specific for ATG12. |
Additional Info
| Post Translational Modifications | Acetylated by EP300. |
| Function | Ubiquitin-like protein involved in autophagy vesicles formation. Conjugation with ATG5 through a ubiquitin-like conjugating system involving also ATG7 as an E1-like activating enzyme and ATG10 as an E2-like conjugating enzyme, is essential for its function. The ATG12-ATG5 conjugate acts as an E3-like enzyme which is required for lipidation of ATG8 family proteins and their association to the vesicle membranes. As part of the ATG8 conjugation system with ATG5 and ATG16L1, required for recruitment of LRRK2 to stressed lysosomes and induction of LRRK2 kinase activity in response to lysosomal stress. (Microbial infection) May act as a proviral factor. In association with ATG5, negatively regulates the innate antiviral immune response by impairing the type I IFN production pathway upon vesicular stomatitis virus (VSV) infection. |
| Protein Name | Ubiquitin-Like Protein Atg12Autophagy-Related Protein 12Apg12-Like |
| Database Links | Reactome: R-MMU-1632852Reactome: -MMU-5205685Reactome: -MMU-8934903 |
| Cellular Localisation | CytoplasmPreautophagosomal Structure MembranePeripheral Membrane ProteinTecpr1 Recruits The Atg12-Atg5 Conjugate To The Autolysosomal Membrane |
| Alternative Antibody Names | Anti-Ubiquitin-Like Protein Atg12 antibodyAnti-Autophagy-Related Protein 12 antibodyAnti-Apg12-Like antibodyAnti-Atg12 antibodyAnti-Apg12 antibodyAnti-Apg12l antibody |
Information sourced from Uniprot.org