• Immunofluorescence analysis of PC-12 cells using ATF6 Rabbit polyclonal antibody (STJ11103832) at a dilution of 1:200 (40x lens). Secondary antibody:Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of PC-12 cells using ATF6 Rabbit polyclonal antibody (STJ11103832) at a dilution of 1:100 (40x lens). Secondary antibody:Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of NIH/3T3 cells using ATF6 Rabbit polyclonal antibody (STJ11103832) at a dilution of 1:100 (40x lens). Secondary antibody:Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of HeLa cells using ATF6 Rabbit polyclonal antibody (STJ11103832) at a dilution of 1:100 (40x lens). Secondary antibody:Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Immunohistochemistry analysis of ATF6 in paraffin-embedded rat kidney tissue using ATF6 Rabbit polyclonal antibody (STJ11103832) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0. 01 M citrate buffer (pH 6. 0) prior to immunohistochemistry staining.
  • Western blot analysis of various lysates using ATF6 Rabbit polyclonal antibody (STJ11103832) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.

Anti-ATF6 antibody (600-670) (STJ11103832)

SKU:
STJ11103832

Current Stock:
Host: Rabbit
Applications: WB/IHC/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-Cyclic Amp-Dependent Transcription Factor Atf-6 Alpha (600-670) is suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IHC-P 1:50-1:200
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: ATF6
Gene ID: 22926
Uniprot ID: ATF6A_HUMAN
Immunogen Region: 600-670
Immunogen: A synthetic peptide corresponding to a sequence within amino acids 600-670 of human ATF6 (NP_031374.2).
Immunogen Sequence: INENVINGQDYEVMMQIDCQ VMDTRILHIKSSSVPPYLRD QQRNQTNTFFGSPPAATEAT HVVSTIPESLQ
Tissue Specificity Ubiquitous.
Post Translational Modifications During unfolded protein response, a fragment of approximately 50 kDa containing the cytoplasmic transcription factor domain is released by proteolysis. The cleavage seems to be performed sequentially by site-1 (MBTPS1, S1P) and site-2 (MBTPS2, S2P) proteases. N-glycosylated. The glycosylation status may serve as a sensor for ER homeostasis, resulting in ATF6 activation to trigger the unfolded protein response (UPR). Ubiquitinated by RNF186 at Lys-152, which is required for pattern recognition receptor-induced unfolded protein response-associated outcomes.
Function Cyclic AMP-dependent transcription factor ATF-6 alpha: Precursor of the transcription factor form (Processed cyclic AMP-dependent transcription factor ATF-6 alpha), which is embedded in the endoplasmic reticulum membrane. Endoplasmic reticulum stress promotes processing of this form, releasing the transcription factor form that translocates into the nucleus, where it activates transcription of genes involved in the unfolded protein response (UPR). Processed cyclic AMP-dependent transcription factor ATF-6 alpha: Transcription factor that initiates the unfolded protein response (UPR) during endoplasmic reticulum stress by activating transcription of genes involved in the UPR. Binds DNA on the 5'-CCACGA-3'half of the ER stress response element (ERSE) (5'-CCAAT-N(9)-CCACGA-3') and of ERSE II (5'-ATTGG-N-CCACG-3'). Binding to ERSE requires binding of NF-Y to ERSE. Could also be involved in activation of transcription by the serum response factor. May play a role in foveal development and cone function in the retina.
Protein Name Cyclic Amp-Dependent Transcription Factor Atf-6 Alpha
Camp-Dependent Transcription Factor Atf-6 Alpha
Activating Transcription Factor 6 Alpha
Atf6-Alpha Cleaved Into - Processed Cyclic Amp-Dependent Transcription Factor Atf-6 Alpha
Database Links Reactome: R-HSA-380994
Reactome: R-HSA-381033
Reactome: R-HSA-381183
Cellular Localisation Endoplasmic Reticulum Membrane
Single-Pass Type Ii Membrane Protein
Golgi Apparatus Membrane
Translocates From The Endoplasmic Reticulum To The Golgi
Where It Is Processed
Processed Cyclic Amp-Dependent Transcription Factor Atf-6 Alpha: Nucleus
Under Er Stress The Cleaved N-Terminal Cytoplasmic Domain Translocates Into The Nucleus
Thbs4 Promotes Its Nuclear Shuttling
Alternative Antibody Names Anti-Cyclic Amp-Dependent Transcription Factor Atf-6 Alpha antibody
Anti-Camp-Dependent Transcription Factor Atf-6 Alpha antibody
Anti-Activating Transcription Factor 6 Alpha antibody
Anti-Atf6-Alpha Cleaved Into - Processed Cyclic Amp-Dependent Transcription Factor Atf-6 Alpha antibody
Anti-ATF6 antibody

Information sourced from Uniprot.org

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