| Host: |
Rabbit |
| Applications: |
WB |
| Reactivity: |
Human/Rat/Mouse |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Rabbit polyclonal antibody anti-Cyclic AMP-dependent transcription factor ATF-6 alpha (221-271 aa) is suitable for use in Western Blot research applications. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide. |
| Purification: |
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Concentration: |
1 mg/mL |
| Dilution Range: |
WB 1:500-2000 |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: |
ATF6 |
| Gene ID: |
22926 |
| Uniprot ID: |
ATF6A_HUMAN |
| Immunogen Region: |
221-271 aa |
| Specificity: |
This antibody detects endogenous levels of ATF6A at Human |
| Immunogen: |
Synthesized peptide derived from the human ATF6A at the amino acid range 221-271 |
| Post Translational Modifications | During unfolded protein response, a fragment of approximately 50 kDa containing the cytoplasmic transcription factor domain is released by proteolysis. The cleavage seems to be performed sequentially by site-1 (MBTPS1, S1P) and site-2 (MBTPS2, S2P) proteases. N-glycosylated. The glycosylation status may serve as a sensor for ER homeostasis, resulting in ATF6 activation to trigger the unfolded protein response (UPR). Ubiquitinated by RNF186 at Lys-152, which is required for pattern recognition receptor-induced unfolded protein response-associated outcomes. |
| Function | Cyclic AMP-dependent transcription factor ATF-6 alpha: Precursor of the transcription factor form (Processed cyclic AMP-dependent transcription factor ATF-6 alpha), which is embedded in the endoplasmic reticulum membrane. Endoplasmic reticulum stress promotes processing of this form, releasing the transcription factor form that translocates into the nucleus, where it activates transcription of genes involved in the unfolded protein response (UPR). Processed cyclic AMP-dependent transcription factor ATF-6 alpha: Transcription factor that initiates the unfolded protein response (UPR) during endoplasmic reticulum stress by activating transcription of genes involved in the UPR. Binds DNA on the 5'-CCACGA-3'half of the ER stress response element (ERSE) (5'-CCAAT-N(9)-CCACGA-3') and of ERSE II (5'-ATTGG-N-CCACG-3'). Binding to ERSE requires binding of NF-Y to ERSE. Could also be involved in activation of transcription by the serum response factor. May play a role in foveal development and cone function in the retina. |
| Protein Name | Cyclic Amp-Dependent Transcription Factor Atf-6 AlphaCamp-Dependent Transcription Factor Atf-6 AlphaActivating Transcription Factor 6 AlphaAtf6-Alpha Cleaved Into - Processed Cyclic Amp-Dependent Transcription Factor Atf-6 Alpha |
| Database Links | Reactome: R-HSA-380994Reactome: R-HSA-381033Reactome: R-HSA-381183 |
| Cellular Localisation | Endoplasmic Reticulum MembraneSingle-Pass Type Ii Membrane ProteinGolgi Apparatus MembraneTranslocates From The Endoplasmic Reticulum To The GolgiWhere It Is ProcessedProcessed Cyclic Amp-Dependent Transcription Factor Atf-6 Alpha: NucleusUnder Er Stress The Cleaved N-Terminal Cytoplasmic Domain Translocates Into The NucleusThbs4 Promotes Its Nuclear Shuttling |
| Alternative Antibody Names | Anti-Cyclic Amp-Dependent Transcription Factor Atf-6 Alpha antibodyAnti-Camp-Dependent Transcription Factor Atf-6 Alpha antibodyAnti-Activating Transcription Factor 6 Alpha antibodyAnti-Atf6-Alpha Cleaved Into - Processed Cyclic Amp-Dependent Transcription Factor Atf-6 Alpha antibodyAnti-ATF6 antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
