• Immunohistochemistry analysis of paraffin-embedded human lung cancer using ATF3 Rabbit polyclonal antibody (STJ115430) at dilution of 1:300 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of extracts of RAW264. 7 cells, using ATF3 antibody (STJ115430) at 1:1000 dilution. Raw264. 7 cells were treated by LPS (1 Mu g/ml) at 37 °C for 8 hours. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.
  • Western blot analysis of extracts of 293T cells, using ATF3 antibody (STJ115430) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Anti-ATF3 antibody (1-181) (STJ115430)

SKU:
STJ115430

£169.50 - £449.50
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Host: Rabbit
Applications: WB/IHC
Reactivity: Human/Mouse
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-ATF3 (1-181) is suitable for use in Western Blot and Immunohistochemistry research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
IHC-P 1:100-1:500
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: ATF3
Gene ID: 467
Uniprot ID: ATF3_HUMAN
Immunogen Region: 1-181
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-181 of human ATF3 (NP_001665.1).
Immunogen Sequence: MMLQHPGQVSASEVSASAIV PCLSPPGSLVFEDFANLTPF VKEELRFAIQNKHLCHRMSS ALESVTVSDRPLGVSITKAE VAPEEDERKKRRRERNKIAA AKCRNKKKEKTECLQKESEK LESVNAELKAQIEELKNEKQ HLIYMLNLHRPTCIVRAQNG RTPEDERNLFIQQIKEGTLQ S
Function This protein binds the cAMP response element (CRE) (consensus: 5'-GTGACGTACAG-3'), a sequence present in many viral and cellular promoters. Represses transcription from promoters with ATF sites. It may repress transcription by stabilizing the binding of inhibitory cofactors at the promoter. Isoform 2: Activates transcription presumably by sequestering inhibitory cofactors away from the promoters. Isoform 3: Stress-induced isoform, counteracts the transcriptional repression of isoform 1.
Protein Name Cyclic Amp-Dependent Transcription Factor Atf-3
Camp-Dependent Transcription Factor Atf-3
Activating Transcription Factor 3
Database Links Reactome: R-HSA-380994
Reactome: R-HSA-9633012
Reactome: R-HSA-9648895
Cellular Localisation Nucleus
Alternative Antibody Names Anti-Cyclic Amp-Dependent Transcription Factor Atf-3 antibody
Anti-Camp-Dependent Transcription Factor Atf-3 antibody
Anti-Activating Transcription Factor 3 antibody
Anti-ATF3 antibody

Information sourced from Uniprot.org

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