• Western blot analysis of extracts of various cell lines, using ATF2 antibody (STJ22702) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.
  • Western blot analysis of extracts from normal (control) and ATF2 rabbit polyclonal antibody knockout (KO) 293T cells, using ATF2 rabbit polyclonal antibody antibody (STJ22702) at 1:500 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.
  • Immunohistochemistry of paraffin-embedded rat brain using ATF2 rabbit polyclonal antibody (STJ22702) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry of paraffin-embedded mouse spinal cord using ATF2 rabbit polyclonal antibody (STJ22702) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.

Anti-ATF2 antibody (110-190) (STJ22702)

SKU:
STJ22702

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Host: Rabbit
Applications: WB/IHC
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-ATF2 (110-190) is suitable for use in Western Blot and Immunohistochemistry research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IHC-P 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: ATF2
Gene ID: 1386
Uniprot ID: ATF2_HUMAN
Immunogen Region: 110-190
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 110-190 of ATF2 (NP_001871.2).
Immunogen Sequence: DLSPLATPIIRSKIEEPSVV ETTHQDSPLPHPESTTSDEK EVPLAQTAQPTSAIVRPASL QVPNVLLTSSDSSVIIQQAV P
Tissue Specificity Ubiquitously expressed, with more abundant expression in the brain.
Post Translational Modifications Phosphorylation of Thr-69 by MAPK14 and MAPK11, and at Thr-71 by MAPK1/ERK2, MAPK3/ERK1, MAPK11, MAPK12 and MAPK14 in response to external stimulus like insulin causes increased transcriptional activity. Phosphorylated by PLK3 following hyperosmotic stress. Also phosphorylated and activated by JNK and CaMK4. ATM-mediated phosphorylation at Ser-490 and Ser-498 stimulates its function in DNA damage response. Phosphorylation at Ser-62, Thr-73 and Ser-121 activates its transcriptional activity. Phosphorylation at Thr-69 or Thr-71 enhances acetylation of histones H2B and H4.
Function Transcriptional activator which regulates the transcription of various genes, including those involved in anti-apoptosis, cell growth, and DNA damage response. Dependent on its binding partner, binds to CRE (cAMP response element) consensus sequences (5'-TGACGTCA-3') or to AP-1 (activator protein 1) consensus sequences (5'-TGACTCA-3'). In the nucleus, contributes to global transcription and the DNA damage response, in addition to specific transcriptional activities that are related to cell development, proliferation and death. In the cytoplasm, interacts with and perturbs HK1- and VDAC1-containing complexes at the mitochondrial outer membrane, thereby impairing mitochondrial membrane potential, inducing mitochondrial leakage and promoting cell death. The phosphorylated form (mediated by ATM) plays a role in the DNA damage response and is involved in the ionizing radiation (IR)-induced S phase checkpoint control and in the recruitment of the MRN complex into the IR-induced foci (IRIF). Exhibits histone acetyltransferase (HAT) activity which specifically acetylates histones H2B and H4 in vitro. In concert with CUL3 and RBX1, promotes the degradation of KAT5 thereby attenuating its ability to acetylate and activate ATM. Can elicit oncogenic or tumor suppressor activities depending on the tissue or cell type.
Protein Name Cyclic Amp-Dependent Transcription Factor Atf-2
Camp-Dependent Transcription Factor Atf-2
Activating Transcription Factor 2
Cyclic Amp-Responsive Element-Binding Protein 2
Creb-2
Camp-Responsive Element-Binding Protein 2
Hb16
Camp Response Element-Binding Protein Cre-Bp1
Database Links Reactome: R-HSA-2151201
Reactome: R-HSA-3214847
Reactome: R-HSA-400253
Reactome: R-HSA-450341
Reactome: R-HSA-6796648
Reactome: R-HSA-8943724
Reactome: R-HSA-9018519
Reactome: R-HSA-9031628
Reactome: R-HSA-9633012
Reactome: R-HSA-9707616
Cellular Localisation Nucleus
Cytoplasm
Mitochondrion Outer Membrane
Shuttles Between The Cytoplasm And The Nucleus And Heterodimerization With Jun Is Essential For The Nuclear Localization
Localization To The Cytoplasm Is Observed Under Conditions Of Cellular Stress And In Disease States
Localizes At The Mitochondrial Outer Membrane In Response To Genotoxic Stress
Phosphorylation At Thr-52 Is Required For Its Nuclear Localization And Negatively Regulates Its Mitochondrial Localization
Co-Localizes With The Mrn Complex In The Ir-Induced Foci (Irif)
Alternative Antibody Names Anti-Cyclic Amp-Dependent Transcription Factor Atf-2 antibody
Anti-Camp-Dependent Transcription Factor Atf-2 antibody
Anti-Activating Transcription Factor 2 antibody
Anti-Cyclic Amp-Responsive Element-Binding Protein 2 antibody
Anti-Creb-2 antibody
Anti-Camp-Responsive Element-Binding Protein 2 antibody
Anti-Hb16 antibody
Anti-Camp Response Element-Binding Protein Cre-Bp1 antibody
Anti-ATF2 antibody
Anti-CREB2 antibody
Anti-CREBP1 antibody

Information sourced from Uniprot.org

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