Anti-ATF1 antibody [SRM15018] (STJ11109748)

SPECIFICATIONS
ClonalityMonoclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 1-89 of human ATF1 (NP_005162.1).
STJ11109748
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General Information

Short DescriptionRabbit monoclonal ATF1 antibody for use in WB, IF, ICC, IHC-P and ELISA in human, mouse and rat samples. Datasheet included with dilution recommendations, and related reagents.
ApplicationsWB/IF/ICC/IHC-P/ELISA
HostRabbit
ReactivityHuman/Mouse/Rat
NoteSTRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.

Product Properties

ClonalityMonoclonal
Clone IDSRM15018
IsotypeIgG
ConjugationUnconjugated
PurificationAffinity purification
Dilution RangeWB, 1:7000-1:14000
IF/ICC, 1:200-1:400
IHC-P, 1:500-1:2000
ELISA, Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements.
Storage InstructionStore at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.

Target Information

Gene SymbolATF1
Gene ID466
Uniprot IDATF1_HUMAN
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 1-89 of human ATF1 (NP_005162.1).

Additional Info

Post Translational Modifications Phosphorylated at Ser-198 by HIPK2 in response to genotoxic stress. This phosphorylation promotes transcription repression of FTH1 and other antioxidant detoxification genes. The CDK3-mediated phosphorylation at Ser-63 promotes its transactivation and transcriptional activities. Phosphorylated at Ser-63 by RPS6KA4 and RPS6KA5 in response to mitogenic or stress stimuli.
Function This protein binds the cAMP response element (CRE) (consensus: 5'-GTGACGTACAG-3'), a sequence present in many viral and cellular promoters. Binds to the Tax-responsive element (TRE) of HTLV-I. Mediates PKA-induced stimulation of CRE-reporter genes. Represses the expression of FTH1 and other antioxidant detoxification genes. Triggers cell proliferation and transformation.
Protein Name Cyclic Amp-Dependent Transcription Factor Atf-1
Camp-Dependent Transcription Factor Atf-1
Activating Transcription Factor 1
Protein Treb36
Database Links Reactome: R-HSA-199920
Reactome: R-HSA-9031628
Cellular Localisation Nucleus
Alternative Antibody Names Anti-Cyclic Amp-Dependent Transcription Factor Atf-1 antibody
Anti-Camp-Dependent Transcription Factor Atf-1 antibody
Anti-Activating Transcription Factor 1 antibody
Anti-Protein Treb36 antibody
Anti-ATF1 antibody

Information sourced from Uniprot.org

Citations

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